丁香实验_LOGO
登录
提问
我要登录
|免费注册
点赞
收藏
wx-share
分享

Assays for Cyclin-Dependent Kinase Inhibitors

互联网

791
Cyclin-dependent kinases (CDK) are serine/threonine kinases that regulate cell cycle control and progression (1 ,2 ). A CDK holoenzyme complex is active if associated with its cyclin partner and if the complex is phosphorylated at specific activating residues (threonine 160/161) (1 ,2 ). The progression through the cell cycle is mediated by the sequential activation of CDKs during different phases of the cycle. The G1/S phase transition of the cell cycle is mediated by phosphorylation of the Retinoblastoma gene product (Rb) by CDK4 and/or CDK6 (G1/S checkpoint), leading to the release of bound E2F from Rb and allowing the transcription of genes by “free E2F” necessary for S phase progression. During late G1 phase, CDK2/cyclin E complex phosphorylates several substrates including Rb. On the other hand, CDK2/cyclin A complex is active throughout S phase. For cells to progress to mitosis, CDK1 (CDC2) must be “activated,” and activated cdc2 phosphorylates substrates required for mitosis (3 ).
提问
扫一扫
丁香实验小程序二维码
实验小助手
丁香实验公众号二维码
扫码领资料
反馈
TOP
打开小程序