Ca2+ Imaging of Dendrites and Spines
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The intracellular calcium concentration is one key parameter triggering numerous intracellular signalling pathways in neuronal
cells. The development of optical techniques like fast confocal or 2-photon microscopy has made it possible to measure calcium
dynamics even in sub-cellular compartments like dendrites and dendritic spines at high temporal and spatial resolution. This
chapter provides experimental and technical details for different imaging techniques appropriate for calcium measurements
in sub-cellular compartments, discusses specific advantages and limitations and calls attention to possible pitfalls.
