Preparation of Recombinant Adenoviruses

Adenoviruses are viruses with genomes of double-stranded DNA approx 36 kb in size. There are more than 100 different serotypes that have been identified, 47 of them of human origin. Human serotypes have been grouped into six subgenera (A-F) and whereas sequence similarity between genera is low, other molecular biologic aspects of the viruses are almost identical. Of all the human adenoviruses, serotypes 2 and 5 (subgenus C) have been the most extensively studied (1 ).