Phage Display Technology: Protocols

Over the past few years, considerable effort has gone into genetic engineering and manipulation of antibody molecules. One consequence of this research has been the use of filamentous phage as a vehicle on which to display antibody fragments. It is possible to express a variety of molecules on the surface of phage, including peptides and antibody fragments. With regard to antibodies, libraries of both sFv and Fab fragments have been successfully cloned onto the surface of bacteriophages, usually via the N terminal of the gene III product, although expression on the gene VIII product has also been employed to isolate low-affinity antibodies.