丁香实验_LOGO
登录
提问
我要登录
|免费注册
点赞
收藏
wx-share
分享

2-DE Spot Amino Acid Analysis with 9-Fluorenylmethyl Chloroformate

互联网

657
Amino acid analysis is a powerful and sensitive technique for the determination of the amino acid composition of proteins. In the past, the automation of amino acid analysis was based on postcolumn ninhydrin analysis, e.g., the Beckman 6300. However, a demand for high sensitivity and high sample throughput has led to the development of precolumn derivatization of amino acids and subsequent separation by reverse phase-high performance liquid chromatography RP-(HPLC), e.g., GBC AMINOMATE system. Although the chromophoric or fluorogenic derivatives allow greater sensitivity (100 fmol to low pmol range of protein), protein hydrolysis remains the limiting factor to high sensitive amino acid analysis. At the 5- to 20-pmol level, various contaminants influence the accuracy of both the qualitative and quantitative aspects of the analysis. Therefore, correct procedure and careful manipulation are required to achieve a successful analysis.
提问
扫一扫
丁香实验小程序二维码
实验小助手
丁香实验公众号二维码
扫码领资料
反馈
TOP
打开小程序