读懂GenBank文件格式中的资料

一、 LOCUS

在 GenBank 格式中，

LOCUS NM_001469 2156 bp mRNA linear （家系血统） PRI （ primate 猿类） 16-DEC-2004
DEFINITION Homo sapiens thyroid autoantigen 70kDa (Ku antigen) (G22P1), mRNA.

The LOCUS field contains a number of different data elements, including locus name, sequence length, molecule type, GenBank division, and modification date. Each element is described below.

二、 COMMENT

1 、 REVIEWED REFSEQ ：说明了该 RefSeq 生成的过程。

2 、 Summary ：说明了该序列的功能。

三、 Feature

名词解释： information about genes and gene products, as well as regions of （ biological significance reported in the ） sequence. These can include regions of the sequence that code for proteins and RNA molecules.

Feature 下的副标题内容太复杂，必要时到这里 The DDBJ/EMBL/GenBank Feature Table 查 .

1 、 key ：一般选择 Location/Qualifier 。

2 、 complement ： cDNA 。 If a feature is located on the complementary strand, the word "complement" will appear before the base span.

3 、 5< ：指向 5 ’端。 If the "<" symbol precedes a base span, the sequence is partial on the 5' end (e.g., CDS <1..206). If the ">" symbol follows a base span, the sequence is partial on the 3' end (e.g., CDS 435..915>.

4 、 /db_xref ：其字符串是通往其他数据库的链接。

/db_xref="taxon:9606" taxonomy 物种分类学
/db_xref="GeneID:2547" 链接到 Gene 。
/db_xref="LocusID:2547" 链接到 Locuslink 。
/db_xref="MIM:152690" 链接到 OMIM 。

四、两个例子：

CDS=23..400
====/product="alcohol dehydrogenase"
====/gene="adhI"
might be read as:
The feature CDS is a coding sequence beginning at base 23 and ending at base 400, has a product called 'alcohol dehydrogenase' and is coded for by a gene called “ adhI ”

A more complex description:
Key=Location/Qualifiers
CDS=join(544..589,688..>1032)
====/product="T-cell receptor beta-chain"
which might be read as:
This feature, which is a partial coding sequence is formed by joining elements indicated to form one contiguous sequence encoding a product called T-cell receptor beta-chain.