Isolate DNA from NRBC Blood from Buccal Swab or dried blood spots collected with filter paper
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实验试剂
1. Absolute Ethanol - approximately 0.3 ml per sample
2. RNase A(Optional) - Prepare a stock of RNase A at 50mg/ml
实验设备
1. Tabletop microcentrifuge and sterile 1.5 ml tubes
实验步骤
2. Add 25ul OB Protease and 500ul Buffer BL into sample. Mix by vortexing at maxi speed for 30s.
3. Incubate at 65°C for 10 minutes. Collect any liquid drop from lid by brief centrifugation.
4. Centrifuge at 14,000 x g for 10 minutes. Carefully transfer the supernatant to a new 1.5 ml tube.