Features of the Husbandry to be Equated Across Labs
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Features of the Husbandry to be Equated Across Labs
Five periods may differ in the requirements to some extent. Features mentioned only for the first period are common to all periods.
After arrival from supplier but before mating; housed with same sex
Mated pairs before and after delivery of litter
Weaned offspring before the start of behavioral testing
Preparation for the formal behavioral testing - may be specific to this study
During behavioral testing
After arrival
Food Purina 5001 (4.5% fat)
Bedding Bed-O-Cob 1/4"
Nesting material none
Cage top Stainless steel bars (plus passive filter top in Portland)
Animal handling Gloved hand
Animal marking nil
Animal weighing nil
Mice/cage 4 or 5 maximum
Lights on/off 0600/1800
Mated pairs (items the same as after arrival are not repeated)
Food Purina 5020 (9% fat)
Nesting material 1 Nestlet
Marking none
Weighing at mating
Mice/cage 1 female, 1 male
Male present with litter? Yes; postpartum pregnancy allowed
Inspection of new litter Count live and dead pups; remove dead, put live in nest
Culling of newborns Remove only the obvious runts for euthanasia
Cage changing with litter Do not disturb cage until litter is 4 days old; then clean cage, including fresh nestlet
Weaned offspring
Age at weaning Aim for 21 days (may be ± 1 day when weekend involved)
Weighing Record body weight to nearest 0.5g; find means for each sex
Food Purina 5001
Mice/cage Same sex littermates, 4 or 5 maximum
Marking nil
About two weeks before the start of behavioral testing
Mice/cage Cull to 2 by random lottery (keep some spares in reserve)
Cage changing day Friday
During behavioral testing
Marking Sharpie pen (black or red) on tail; start Monday AM
Weighing On Friday before the coke test
Handling Hand with plastic surgical glove
Cage changing day Friday (AFTER coke test trial for each pair)
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