Gel Mobility Shift Analysis

Material/reagents:

• 2X binding buffer:
      20 mM Tris pH 7.5 
      100 mM NaCl 
      2 mM EDTA 
      10% glycerol

• poly(dI-dC) (5 mg/ml in TE)

• BSA (10 mg/ml)

• 32P-labeled probe

• Buffer C: 
      20 mM HEPES pH 7.9 
      25% glycerol 
      420 mM NaCl 
      1.5 mM MgCl2

• 4% native polyacrylamide gel (50 ml): 
      5 ml 40% acrylamide (30:1) 
      2.5 ml 5X TBE 
      41.95 ml H20 
      0.5 ml 10% APS 
      50 ml TEMED

• 0.25X TBE running buffer

• Loading dye: 
      0.2% bromophenol blue 
      0.2% xylene cyanol 
      50% glycerol 
      0.5 mM DTT 
      0.5 mM PMSF 
      0.2 mM EDTA

具体操作流程请点击下面的链接下载：

Gel Mobility Shift Analysis .pdf