验证引物
丁香园论坛
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我做heparanaser的RT-PCR,请各位帮助验证引物
cDNA序列为:
1 atgctgctgc gctcgaagcc tgcgctgccg ccgccgctgc tgatgctgct gctcctgggg
61 ccgctgggtc ccctctcccc tggcgccctg ccccgacctg cgcaagcaca gcaggacgtc
121 gtggacctgg acttcttcac ccaggagccg ctgcacctgg tgagcccctc gttcctgtcc
181 gtcaccattg acgccaacct ggccacggac ccgcggttcc tcatcctcct gggttctcca
241 aagcttcgta ccttggccag aggcttgtct cctgcgtacc tgaggtttgg tggcaccaag
301 acagacttcc taattttcga tcccaagaag gaatcaacct ttgaagagag aagttactgg
361 caatctcaag tcaaccagga tatttgcaaa tatggatcca tccctcctga tgtggaggag
421 aagttacggt tggaatggcc ctaccaggag caattgctac tccgagaaca ctaccagaaa
481 aagttcaaga acagcaccta ctcaagaagc tctgtagatg tgctatacac ttttgcaaac
541 tgctcaggac tggacttgat ctttggccta aatgcgttat taagaacagc agatttgcag
601 tggaacagtt ctaatgctca gttgctcctg gactactgct cttccaaggg gtataacatt
661 tcttgggaac taggcaatga acctaacagt ttccttaaga aggctgatat tttcatcaat
721 gggtcgcagt taggagaaga ttttattcaa ttgcataaac ttctaagaaa gtccaccttc
781 aaaaatgcaa aactctatgg tcctgatgtt ggtcagcctc gaagaaagac ggctaagatg
841 ctgaagagct tcctgaaggc tggtggagaa gtgattgatt cagttacatg gcatcactac
901 tatttgaatg gacggactgc taccagggaa gattttctaa accctgatgt attggacatt
961 tttatttcat ctgtgcaaaa agttttccag gtggttgaga gcaccaggcc tggcaagaag
1021 gtctggttag gagaaacaag ctctgcatat ggaggcggag cgcccttgct atccgacacc
1081 tttgcagctg gctttatgtg gctggataaa ttgggcctgt cagcccgaat gggaatagaa
1141 gtggtgatga ggcaagtatt ctttggagca ggaaactacc atttagtgga tgaaaacttc
1201 gatcctttac ctgattattg gctatctctt ctgttcaaga aattggtggg caccaaggtg
1261 ttaatggcaa gcgtgcaagg ttcaaagaga aggaagcttc gagtatacct tcattgcaca
1321 aacactgaca atccaaggta taaagaagga gatttaactc tgtatgccat aaacctccat
1381 aacgtcacca agtacttgcg gttaccctat cctttttcta acaagcaagt ggataaatac
1441 cttctaagac ctttgggacc tcatggatta ctttccaaat ctgtccaact caatggtcta
1501 actctaaaga tggtggatga tcaaaccttg ccacctttaa tggaaaaacc tctccggcca
1561 ggaagttcac tgggcttgcc agctttctca tatagttttt ttgtgataag aaatgccaaa
1621 gttgctgctt gcatctgaaa ataaaatata ctagtcctga cactgaaaa
AF084467-A (bp905): 5'-TGAATGGACGGACTGCTACC-3'
AF084467-B (bp1153C): 5'-GCCTCATCACCACTTCTATTCC-3'
product:249bps
B-actin-A (bp2281): 5'-CGGGAAATCGTGCGTGACA-3'
B-actin-B (bp2963C): 5'-TCGTCATACTCCTGCTTGCTG-3'
product:683bps
cDNA序列为:
1 atgctgctgc gctcgaagcc tgcgctgccg ccgccgctgc tgatgctgct gctcctgggg
61 ccgctgggtc ccctctcccc tggcgccctg ccccgacctg cgcaagcaca gcaggacgtc
121 gtggacctgg acttcttcac ccaggagccg ctgcacctgg tgagcccctc gttcctgtcc
181 gtcaccattg acgccaacct ggccacggac ccgcggttcc tcatcctcct gggttctcca
241 aagcttcgta ccttggccag aggcttgtct cctgcgtacc tgaggtttgg tggcaccaag
301 acagacttcc taattttcga tcccaagaag gaatcaacct ttgaagagag aagttactgg
361 caatctcaag tcaaccagga tatttgcaaa tatggatcca tccctcctga tgtggaggag
421 aagttacggt tggaatggcc ctaccaggag caattgctac tccgagaaca ctaccagaaa
481 aagttcaaga acagcaccta ctcaagaagc tctgtagatg tgctatacac ttttgcaaac
541 tgctcaggac tggacttgat ctttggccta aatgcgttat taagaacagc agatttgcag
601 tggaacagtt ctaatgctca gttgctcctg gactactgct cttccaaggg gtataacatt
661 tcttgggaac taggcaatga acctaacagt ttccttaaga aggctgatat tttcatcaat
721 gggtcgcagt taggagaaga ttttattcaa ttgcataaac ttctaagaaa gtccaccttc
781 aaaaatgcaa aactctatgg tcctgatgtt ggtcagcctc gaagaaagac ggctaagatg
841 ctgaagagct tcctgaaggc tggtggagaa gtgattgatt cagttacatg gcatcactac
901 tatttgaatg gacggactgc taccagggaa gattttctaa accctgatgt attggacatt
961 tttatttcat ctgtgcaaaa agttttccag gtggttgaga gcaccaggcc tggcaagaag
1021 gtctggttag gagaaacaag ctctgcatat ggaggcggag cgcccttgct atccgacacc
1081 tttgcagctg gctttatgtg gctggataaa ttgggcctgt cagcccgaat gggaatagaa
1141 gtggtgatga ggcaagtatt ctttggagca ggaaactacc atttagtgga tgaaaacttc
1201 gatcctttac ctgattattg gctatctctt ctgttcaaga aattggtggg caccaaggtg
1261 ttaatggcaa gcgtgcaagg ttcaaagaga aggaagcttc gagtatacct tcattgcaca
1321 aacactgaca atccaaggta taaagaagga gatttaactc tgtatgccat aaacctccat
1381 aacgtcacca agtacttgcg gttaccctat cctttttcta acaagcaagt ggataaatac
1441 cttctaagac ctttgggacc tcatggatta ctttccaaat ctgtccaact caatggtcta
1501 actctaaaga tggtggatga tcaaaccttg ccacctttaa tggaaaaacc tctccggcca
1561 ggaagttcac tgggcttgcc agctttctca tatagttttt ttgtgataag aaatgccaaa
1621 gttgctgctt gcatctgaaa ataaaatata ctagtcctga cactgaaaa
AF084467-A (bp905): 5'-TGAATGGACGGACTGCTACC-3'
AF084467-B (bp1153C): 5'-GCCTCATCACCACTTCTATTCC-3'
product:249bps
B-actin-A (bp2281): 5'-CGGGAAATCGTGCGTGACA-3'
B-actin-B (bp2963C): 5'-TCGTCATACTCCTGCTTGCTG-3'
product:683bps
