Antigen Discovery Using Whole-Genome Phage Display Libraries
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In the last two decades phage display technology has been used for investigating complex biological processes and isolating molecules of practical value in several applications. Bacteriophage lambda, representing a classical cloning and expression system, has also been exploited for generating display libraries of small peptides and protein domains. More recently, large cDNA and whole-genome lambda-display libraries of human pathogens have been generated for the discovery of new antigens for biomedical applications. Here, we describe the construction of a whole-genome library of a common pathogen—Streptococcus pneumoniae —and the use of this library for the molecular dissection of the human B-cell response against bacterial infection and colonization.