Promoters to Express Cloned Genes Uniformly in Drosophila
互联网
366
Forcing ubiquitous expression of a given gene in transgenic Drosophila has become a powerful tool of molecular genetics. Gene misexpression usually causes a dominant phenotype and one may deduce potential functions for the gene from an analysis of this phenotype. Evidently, when a dominant phenotype arises, uniform expression must be inducible such that the ubiquitously expressing construct can be maintained in a silent state in the transgenic stock. Even more informative than uniform expression throughout the animal, is expression in a large patch of tissue, especially when the gene considered acts in a non-cell-autonomous fashion. Here we review the methods available to achieve uniform expression of any cloned gene in Drosophila . We then go on to discuss how expression can be made conditional and/or in large patches of tissue.
![NTERA-2 cl.D1[NT2/D1; NT2D1; Nt2D1; NTERA-2 clone D1; NTERA2-cloneD1; NTERA-2 cl.D1; NTera2 cl.D1; NTERA-2cl.D1; NTera 2/cl.D1; NTERA-2/D1; NTera2/D1; NTERA2-D1; NTera-2D1; Ntera2/D1; NTERA2/D1; NTERA2D1; NTera2D1]人睾丸癌细胞](https://img1.dxycdn.com/p/s14/2024/0726/204/4437331409580366181.jpg!wh200)
