丁香实验_LOGO
登录
提问
我要登录
|免费注册
点赞
收藏
wx-share
分享

Activation of Oocytes After Nuclear Transfer

互联网

467
After nuclear transfer, the recipient oocyte must be stimulated to initiate development. This stimulation is achieved by inducing changes in the oocyte cytoplasm that normally are triggered by the sperm during fertilization. In most cases, such changes include a transient increase in the intracellular-free calcium concentration induced by an electrical pulse or alternatively, by chemical agents. Many times, particularly in aged oocytes, this calcium signal is sufficient to stimulate the oocyte developmental program. Other activation protocols were designed to target pathways downstream of the initial calcium signal to affect the activity of regulatory proteins that play central roles in maintaining developmental arrest. This is achieved by the application of protein kinase or protein synthesis inhibitors; combined with a calcium stimulus such inhibitors are widely used for oocyte activation after nuclear transfer and are able to support embryonic development to term.
提问
扫一扫
丁香实验小程序二维码
实验小助手
丁香实验公众号二维码
扫码领资料
反馈
TOP
打开小程序