Radiolabeling of DNA Using Polynucleotide Kinase

互联网2014-02-08

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Labeling of DNA fragments at the 5′ end with polynucleotide kinase is one of the methods currently employed to obtain highly labeled DNA for sequencing purposes. This end-labeled DNA is used in the partial chemical degradation method for sequencing DNA (see Chapter 51 and ref. 1 ).

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