丁香实验_LOGO
登录
提问
我要登录
|免费注册
点赞
收藏
wx-share
分享

Cosmid Library Construction

互联网

620
To study the organization and regulation of eucaryotic genes from cloned DNA, vectors capable of maintaining large inserts have been constructed, largely from bacteriophage λ, where nonessential intergenic regions were identified. It had been noted that only λ DNA 37–52 kb in length (or 78–102% of the wild-type length) were efficiently packaged and passaged in vivo. Thus vectors from which the intergenic region had been removed could be positively selected for by the insertion of target DNA, thus restoring a packagable DNA length and producing viable phage.
提问
扫一扫
丁香实验小程序二维码
实验小助手
丁香实验公众号二维码
扫码领资料
反馈
TOP
打开小程序