Protocol Protein Gel Staining Using Coomassie Blue Protein Stain
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Materials
- Polyacrylamide pre-cast gel
- SimplyBlue™ Coomassie protein stain, 1x pre-mixed solution (Coomassie blue dye binds to proteins (arginine, the aromatic amino acids, and histidine) allowing protein bands to be visualized in polyacrylamide gels.)
- Plastic container
- Gel separator
Procedure
- After electrophoresis, remove the gel from the gel box and plates using a gel separator
- Place it in a clean plastic container and cover with ultrapure water
- Microwave the gel on high for 1 minute until the solution almost boils (do not overheat)
- Shake the gel on a shaker for 2 minute and discard the water
- Repeat steps 3 and 4 two more times
- After the last wash, add 30 ml of SimplyBlue™ Coomassie protein stain and microwave on high for 45 seconds to 1 minute until the solution almost boils
- Shake the gel on a shaker for 10 minutes
- Wash the gel in 100 ml of ultrapure water for 10 minutes on a shaker
- Add 20 ml of 20% NaCl for at least 5 minutes
- Examine gel on a white paper towel for blue-stained protein bands
- Discard the gel in the Biohazard waste bin
