Bioluminescence Detection for ATP Quantification Using Microfluidic Device

An IISA-ATP (integrated in situ analyzer for ATP) was developed for the quantitative determination of ATP (adenosine-5′-triphosphate) in ocean environments, including the deep-sea. Total (intracellular and dissolved) ATP was quantitatively determined using a simple luciferin-luciferase (L-L) bioluminescence assay. A microfluidic device was incorporated as a core functional element in the IISA-ATP for performing cell lysis and carrying out the L-L reaction. The IISA-ATP has the capability to process environmental samples in the laboratory and in situ. Here, we provide an overview of the performance of the microfluidic device and the IISA-ATP on the basis of results of an in situ trial.