Safe and Effective Mouse Footpad Inoculation

Footpad injection is an important route of inoculation in mouse models of disease and immunology. Although commonly used to deliver antigens as a means of eliciting an efficacious immunological response, herein, we describe a protocol for inoculating mice via footpad injection using a hands-free method to deliver infectious material. These procedures allow for efficient delivery of infectious agents in a manner that is safe for both the researcher and animal.