AFLP Fingerprinting of Arabidopsis
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AFLP− (KeyGene, Wagenmgen, The Netherlands), is a DNA fingerprmtmg technique that visualizes DNA restriction fragments by polymerase chain reaction (PCR) amphfication (1 ,2 ). Routinely, 50-100 restriction fragments are amphfied simultaneously and detected on denaturing polyacrylamide gels. The AFLP technique consists of three major steps:
