Biochemical and Mass Spectrometric Analysis of 3-End Methylation of piRNAs
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Piwi-interacting RNAs (piRNA) are fully modified by 2′-O -methylation at their 3′-termini. This terminal methylation is required to prevent 3′-nucleotide addition, which serves as a tag for destabilization. In this chapter, we describe biochemical and mass spectrometric analyses of 2′-O -methylation at 3′-termini of piRNAs.
