试剂配方:质粒抽提试剂

Solution Ι
Cell resuspension solution (50 mM Tris-HCl, pH 7.5, 10 mM EDTA , RNase A 100 μg/ml)
1 M Tris-HCl (pH 7.6) 2.5 ml
0.25 M EDTA 2.0 ml
ddH ₂ O 45 ml
sterile by autoclave
add 1% RNase 0.5ml
store at room temperature

Solution Ⅱ
Cell lysis solution (0.2 N NaOH, 1% SDS )
Mix 0.4 N NaOH and 2% SDS in same volume.

Solution Ⅲ
Neutralization solution (1.32 M potassium acetate, pH 5.2)
5 M potassium acetate 13.2 ml
Hac 27 ml
adjust pH to 5.2
add ddH ₂ O to 50 ml
sterile by autoclave
store at room temperature

RNaseA: 10mg/ml溶于TE中，在沸水中煮10－30分钟之后分成小份储存于－20℃