磷酸盐缓冲液（PB）的配制
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1. A液（0.2M磷酸二氢钠水溶液）： NaH2 PO4 ・H2 O 27.6g，溶于蒸馏水中，稀释至1000ml。
2. B液（0.2M磷酸氢二钠水溶液）：Na2 HPO4 ・7H2 O 53.6g (或Na2 HPO4 ・12 H2 O 71.6g或Na2 HPO4 ・2 H2 O 35.6g ) 加蒸馏水溶解，加水至1000ml。
3. 不同pH值磷酸盐缓冲液（PB）缓冲液的配制
A液X ml (参照下表) 中，加入B液Y ml，为0.2M PB。若再加蒸馏水至200ml则成0.1M PB。
|
PH
|
X ml
|
Y ml
|
pH
|
X ml
|
Y ml
|
|
5.7
|
93.5
|
6.5
|
6.9
|
45.0
|
55.0
|
|
5.8
|
92.0
|
8.0
|
7.0
|
39.0
|
61.0
|
|
5.9
|
90.0
|
10.0
|
7.1
|
33.0
|
67.0
|
|
6.0
|
87.7
|
12.3
|
7.2
|
28.0
|
72.0
|
|
6.1
|
85.0
|
15.0
|
7.3
|
23.0
|
77.0
|
|
6.2
|
81.5
|
18.5
|
7.4
|
19.0
|
81.0
|
|
6.3
|
77.5
|
22.5
|
7.5
|
16.0
|
84.0
|
|
6.4
|
73.5
|
26.5
|
7.6
|
13.0
|
87.0
|
|
6.5
|
68.5
|
31.5
|
7.7
|
10.0
|
90.0
|
|
6.6
|
62.5
|
37.5
|
7.8
|
8.5
|
91.5
|
|
6.7
|
56.5
|
43.5
|
7.9
|
7.0
|
93.0
|
|
6.8
|
51.0
|
49.0
|
8.0
|
5.3
|
94.7
|
若先分别配成0.1M，1/15M 和1/200 M的A液和B液，可按下表配成相应浓度所需pH值的PB
|
KH2 PO4 或NaH2 PO4 H2 O (ml )
|
Na2 HPO4 (ml )
|
pH ( M/10 )
|
pH( M/15 )
|
pH( M/200 )
|
|
46
|
4
|
5.67
|
5.74
|
5.94
|
|
45
|
5
|
5.78
|
5.83
|
6.06
|
|
44
|
6
|
5.86
|
5.91
|
6.14
|
|
43
|
7
|
5.94
|
5.99
|
6.22
|
|
42
|
8
|
6.02
|
6.07
|
6.30
|
|
41
|
9
|
6.08
|
6.14
|
6.36
|
|
40
|
10
|
6.12
|
6.19
|
6.42
|
|
39
|
11
|
6.17
|
6.24
|
6.47
|
|
38
|
12
|
6.23
|
6.28
|
6.57
|
|
37
|
13
|
6.28
|
6.32
|
6.61
|
|
36
|
14
|
6.33
|
6.37
|
6.65
|
|
35
|
15
|
6.39
|
6.41
|
6.68
|
|
34
|
16
|
6.41
|
6.45
|
6.72
|
|
33
|
17
|
6.45
|
6.49
|
6.75
|
|
32
|
18
|
6.49
|
6.53
|
6.79
|
|
31
|
19
|
6.53
|
6.56
|
6.82
|
|
30
|
20
|
6.55
|
6.59
|
6.88
|
|
29
|
21
|
6.58
|
6.63
|
6.91
|
|
28
|
22
|
6.61
|
6.68
|
6.95
|
|
27
|
23
|
6.65
|
6.72
|
7.00
|
|
26
|
24
|
6.70
|
6.76
|
7.05
|
|
25
|
25
|
6.76
|
6.81
|
7.09
|
|
24
|
26
|
6.81
|
6.86
|
7.13
|
|
23
|
27
|
6.84
|
6.91
|
7.16
|
|
22
|
28
|
6.89
|
6.94
|
7.18
|
|
21
|
29
|
6.89
|
6.96
|
7.20
|
|
20
|
30
|
6.91
|
6.98
|
7.22
|
|
19
|
31
|
6.94
|
7.01
|
7.25
|
|
18
|
32
|
6.97
|
7.03
|
7.26
|
|
17
|
33
|
7.00
|
7.05
|
7.31
|
|
16
|
34
|
7.02
|
7.07
|
7.34
|
|
15
|
35
|
7.06
|
7.11
|
7.38
|
|
14
|
36
|
7.10
|
7.15
|
7.41
|
|
13
|
37
|
7.14
|
7.20
|
7.45
|
|
12
|
38
|
7.17
|
7.24
|
7.45
|
|
11
|
39
|
7.24
|
7.28
|
7.51
|
|
10
|
40
|
7.30
|
7.33
|
7.59
|
|
9
|
41
|
7.36
|
7.40
|
7.65
|
|
8
|
42
|
7.42
|
7.49
|
7.70
|
|
7
|
43
|
7.49
|
7.54
|
7.75
|
|
6
|
44
|
7.57
|
7.61
|
7.80
|
|
5
|
45
|
7.65
|
7.69
|
7.87
|
|
3
|
47
|
7.81
|
7.85
|
8.05
|
|
2
|
48
|
7.92
|
7.97
|
8.15
|
