细胞活性测定

 

<center> <div> <div> <div> <div> <table> <tbody> <tr> <td> <div> <p>  </p> <p> <strong><span><span><span><span></span></span></span></span></strong></p> <p class="MsoNormal"> <strong><span><span><span><b><span>1. 4</span> </b> <b><span>细胞活性测定</span> </b> 原理：</span> </span></span></strong></p> <p class="MsoNormal"> <strong><span><span><span>根据活细胞线粒体内的脱氢酶可将试剂中的有效成分转变为有颜色的<span>Formazan</span> ，并可被酶标仪检测，间接反应活细胞数量。对于细胞增殖、凋亡、生长受抑等可通过细胞数量间接得知的生物学改变，采用细胞活性测定可以提供可应用和参考的数据。</span> </span></span></strong></p> <p class="MsoNormal"> <strong><span><span><span>应用：</span> </span></span></strong></p> <p class="MsoNormal"> <strong><span><span><span>用于生物活性因子的活性检测、大规模药物筛选、细胞增殖、细胞毒性、药敏实验等</span> </span></span></strong></p> <p class="MsoNormal"> <strong><span><span><span>一般流程：</span> </span></span></strong></p> <p class="MsoNormal"> <strong><span><span><span><span>1）<span>   </span> </span> </span> <span>细胞种于<span>96</span> 孔板中，按照客户需要进行各种处理</span> </span></span></strong></p> <p class="MsoNormal"> <strong><span><span><span><span>2）<span>   </span> </span> </span> <span>加入检测试剂并按试剂要求操作</span> </span></span></strong></p> <p class="MsoNormal"> <strong><span><span><span><span>3）<span>   </span> </span> </span> <span>酶标仪检测不同波长处样本的吸光值</span> </span></span></strong></p> <p class="MsoNormal"> <strong><span><span><span><span>4）<span>   </span> </span> </span> <span>数据统计</span> </span></span></strong></p> <p class="MsoNormal"> <strong><span><span><span>基础准备：</span> </span></span></strong></p> <p class="MsoNormal"> <strong><span><span><span><span>l<span>          </span> </span> </span> <span>检测用细胞</span> </span></span></strong></p> <p class="MsoNormal"> <strong><span><span><span><span><span><strong><font>2</font> </strong>       </span> </span> </span> <span>处理方法和处理试剂</span> </span></span></strong></p> <p class="MsoNormal"> <strong><span><span><span>后期结果</span> </span></span></strong></p> <p class="MsoNormal"> <strong><span><span><span>实验原始数据和分析后统计结果</span> </span></span></strong></p> <p class="MsoNormal"> <strong><span><span><span> </span></span></span></strong></p> <p class="MsoNormal"> <strong><span><span>http://www.research-bio.com/technology.asp?id=198&title=细胞技术服务</span></span></strong></p> <strong><span></span> </strong> <p>  </p> <p>  </p> </div> </td> </tr> </tbody> </table> </div> </div> </div> </div> </center>