高速逆流色谱研究进展

<font><strong><font>摘　要：</font></strong>综述了近年来高速逆流色谱(HSCCC)在分析、半制备和制备分离天然产物、蛋白质、抗生素、无机物等领域的研究和应用进展，总结了适用于HSCCC的溶剂体系，并展望了HSCCC与质谱联用、pH区带逆流色谱和离子对逆流色谱新技术的应用前景。</font>

<font><br /><strong>关键词：</strong>高速逆流色谱，溶剂选择，天然产物，抗生素，蛋白质，无机物，质谱，pH区带逆流色谱，离子对逆流色谱，评述</font>

<font><br /><strong>分类号：</strong>O65<strong>　文献标识码：</strong>A</font>

<font>Recent Development in High-speed Countercurrent Chromatography</font>

<font>Dai Deshun</font><font>（</font><font>Analysis Center,Tsinghua University,Beijing 100084</font><font>）　<br /></font><font>Wang Yiming</font><font>（</font><font>Analysis Center,Tsinghua University,Beijing 100084</font><font>）　<br /></font><font>Luo Guoan</font><font>（</font><font>Analysis Center,Tsinghua University,Beijing 100084</font><font>）　<br /></font>

<font><font>Abstract</font></font><font>：</font><font>This paper reviews recent development and applications of high-speed countercurrent chromatography (HSCCC) in the separations on the analytical scale,the semi-preparative and preparative scale of natural products,antibiotics,proteins,inorganic compounds etc.Several series of two-phase solvent systems to facilitate the systematic search suitable for HSCCC are introduced.And the new countercurrent chromatography (CCC)technologies such as HSCCC coupled with MS,pH-zone-refining countercurrent chromatography,and ion-pairing Countercurrent Chromatography are explained and prospected in term of applications.<br /><strong>Keywords</strong></font><font>：</font><font>High-speed countercurrent chromatography,solvent selection,natural products,antibiotics,proteins,inorganic compounds,mass spectrometry,pH-zone-refining countercurrent chromatography,ion-pairing countercurrent chromatography,review</font>

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<font>收稿日期：2000年4月17日</font>

<font>修稿日期：2000年10月17日</font>

<font>出版日期：2001年5月20日</font>