丁香实验_LOGO
登录
提问
提问
我要登录
|免费注册
点赞
收藏
wx-share
分享

Microcal itc200维修与保养

互联网

1462

 

Microcal itc200保养,Microcal itc200维修与保养,Microcal itc200如何保养

 

等温滴定量热仪(ITC)在单次实验中即可同时测定所有结合的热力学参数(n,K,ΔH 和 ΔS)。可应用于检定蛋白、抗体、核酸、脂类和其他生物分子间的相互作用;先导药物的优化;酶促反应动力学以及分子结构修饰改变后的结合效果评估等。由于ITC 不仅仅可用于测定分子间的结合亲和力,更能详细的阐述分子间相互作用的机制,提供机构分析必须的热力学参数,因此该方法正越来越多的被应用于生物分子相互作用的研究。
不仅仅是亲和力:一次试验即可同时确定所有的结合参数,提供从其它结合试验无法获取的所有信息
真实溶解中技术:无需标记或固定。 无缓冲液约束。 易于处理浑浊溶液
应用普适性:高灵敏度地研究所有生物分子反应情况
测量使用竞争性结合技术的 nanomolar 与 picomolar 的结合常数

对Microcal itc200进行定期保养可以提高实验质量进而获得更精确

的实验结果。细胞的清洁度对数据质量有明显的影响。

After every run

The system should be cleaned after each run using the Cell and Syringe Wash command.

Weekly

Clean the cell(s)with 20% Contrad 70 (or 14% Decon 90).Use theDetergent Soak and Rinse (long) command and follow

the instructions to load the cellwith 20% Contrad 70 (or 14% Decon 90) and heatfor 30 minutes at 60°C. This should be done

whenever awaterrinse is insufficientto clean the cell properly. The easiestway to check ifthe cell needs cleaning is to check

thatthe baseline position is no more than 1 μcal/sec lowerthan the reference power setting in the ITCexperiment set up.

Refillthe reference cell with de-ionized water.

Visually inspectthe wash module while performing cell or syringe washing. Verify liquid is flowing to the waste bottle.

Monthly

Inspectthe titration syringe carefully for breaks in the glass. Replace the syringe if it shows any sign of damage. Also inspect

the FPA tip(s) for wear.

Clean the titration syringe afterreverse titrations (protein is loaded into the syringe) or monthly as preventive maintenance.

Pull 2 × 100 μl of 20% Contrad 70 (or 14% Decon 90) into the syringe and then rinse with water using Syringe rinse long on

the Accessory Station.

Every 300 runs, orif it shows signs of wear,replace the plungertip

提问
扫一扫
丁香实验小程序二维码
实验小助手
丁香实验公众号二维码
关注公众号
反馈
TOP
打开小程序