一些关于PCR的网站

一个PCR相关的专业网站，内容很全
http://www.pcrlinks.com/

实验方法与步骤详解
http://swjsx.ntmc.edu.cn/Protocol/home.htm

美国AXYgen公司
http://www.axygen.com/NewFiles/pcr.html

http://www.alkami.com/
This 158 page PCR manual covers the following topics
PCR Primer design
PCR Methods
PCR Polymerases
PCR Variables
PCR Troubleshooting
Special PCR Topics
Appendixes and a comprehensive Index

BioGuide - PCR
http://bip.weizmann.ac.il/mb/bioguide/pcr/contents.html
魏茨曼基因组和生物信息学研究所的一个网页，介绍PCR的初步知识。
包括：
What is PCR?
What are some good reference books for PCR?
How should I select a set of primers to use for PCR?
Programs for designing PCR primers
What is "Hot-start" PCR?
What is AP-PCR or RAPD PCR?
What is "Touchdown" PCR?
Is there a simple method to sequence lambda, M13, or plasmid clones using PCR?

A Look at PCR Cloning Tools
http://www.bioon.com/bbs/post/view?bid=67&id=151321&sty=1&tpg=63&age=0

PCR常见问题及回答
http://www.invitrogen.com.cn/techtitle/main/faq_PCR.htm#2

PCR和Realtime PCR的原理讲的很透, 一个介绍Realtime PCR的教学网站，里面的powerpoint文件非常好
http://www.med.sc.edu:85/pcr/realtime-home.htm

SYBR Green PCR
http://www.bioon.com/bbs/post/view?bid=67&id=638781&sty=1&tpg=5&ppg=1&age=0#638781

PCR QUICK GUIDE
http://www.wzw.tum.de/gene-quantification/quick-guide-pcr.pdfhttp://www.sfb363.uni-halle.de/mod-path/differential_display/sturtevant00.pdfhttp://www.wooster.edu/biology/courses/bio309/default.html

PCR拼接
http://www.bioon.com/bbs/post/view?bid=67&id=305337&sty=1&tpg=47&age=0

知道蛋白如何寻找其氨基酸序列
http://www.bioon.com/bbs/post/view?bid=67&id=401195&sty=1&tpg=36&age=0
http://us.expasy.org/cgi-bin/niceprot.pl?P08253

关于DNA的flash版和文字版
http://www.pbs.org/wgbh/nova/genome/dna.html#

ABI Prism 7000 简要使用说明
http://www.genomultix.com/tech/7000.htm

好书推荐：Practical Protocols of Gene Engineering
http://www.estarray.org/books/

http://www.life.uiuc.edu/bio100/lectures/s02lects/18s02biotechclonerecomb.html
Website dealing with cloning and molecular analysis of genes
http://www.ndsu.nodak.edu/instruct/mcclean/plsc431/cloning/clone3.htm
Some really wonderful illustrations of the techniques of genetic engineering!
http://www.uic.edu/classes/phar/phar331/lecture6/
A truly amazing global resource page on cloning, stem cell research, and related ethical issues, including recent news articles and legislative updates
http://genomics.phrma.org/cloning.html
Animation and article on interspecies cloning
http://www.brookscole.com/chemistry_d/templates/student_resources/0030244269_campbell/HotTopics/InterspeciesCloning.html
From PBS...an animation that illustrates how stem cells are cultivated
http://www.pbs.org/newshour/health/stem_cells.swf
Animations illustrating the use of viruses as vectors for gene therapy
http://www.technologyreview.com/articles/5940.htm
Animations of techniques used for cloning mammals, as well as a create-your-own-clone link
http://library.thinkquest.org/24355/data/details/media/index.html
Another animation of PCR
http://www.people.virginia.edu/~rjh9u/pcranim.html
Animations of restriction digests and gel electrophoresis
http://www.biology.ucsc.edu/classes/bio20L/animate

Gel electrophoresis techniques and simulations
A wonderful website that includes links to a virtual lab on agarose gel electrophoresis:
http://arbl.cvmbs.colostate.edu/hbooks/genetics/biotech/gels/
A Massachusetts Institute of Technology website dealing with Southerns, Northerns, Westerns, and cloning:
http://web.mit.edu/esgbio/www/rdna/rdna.html
2-D gel electrophoresis of proteins:
http://www.fitzscipress.com/Chap05.pdf
A website containing lots of links to electrophoresis techniques and simulations:
http://biobenchelper.hypermart.net/pr/electrophoresis.htm
Want to learn more about chemical bonds? Check out this website:
http://hyperphysics.phy-astr.gsu.edu/hbase/chemical/bond.html

多个录象-多个著名公司
http://www.biocompare.com/video.asp

PCR和 RFLP动画片
http://medtech.cls.msu.edu/ISL/immunology/pcr.htmhttp://medtech.cls.msu.edu/ISL/immunology/rflp.htm

redasoft visual cloning 2000 patch
http://www.bioon.com/bbs/post/view?bid=67&id=496454&sty=1&tpg=26&age=0

分子生物学屏幕保护程序下载
pcr
http://biosoft.biosino.org/down/PCR.exe
蛋白
http://biosoft.biosion.org/down/ssaver.zip
amavi100
http://ourworld.compuserve.com/homepages/esmsoftware/amavi100.zip
dna旋转
http://www.rocketdownload.com/dnload/dna.exe
ddnabase
http://www.rocketdownload.com/dnload/ddnabase.exe
cyclovir
http://mama.indstate.edu/pfaffle/software/cyclovir.exe

http://biosoft.biosino.org/down/source.exehttp://ftp.monash.edu.au//pub/win.galttech/gtmolecules.zip

分子生物学相关软件
http://gene.tmmu.com.cn/biosoft/content.html
１.DNA分析：数据库GenBank, EMBL, DDBJ 查询软件；用来帮助进行PCR引物设计与基因探针设计的软件；功能很全面的DNA序列分析工具包；DNA分析与绘图软件,可绘制线性或环形DNA图；限制酶消化工具；以Java语言写成的序列比较查看器
２.RNA分析：RNA二级结构分析软件；预测RNA二级结构图
３.蛋白质分析：蛋白序列分析软件包；研究膜蛋白拓扑学结构和其它特性的JAVA软件
４.生化教学：生化基础概念演示教学程序； 生化教学文件
５.生化工程：生物反应器（发酵罐）设计软件；发酵实时模拟软件；计算机辅助设计特别是生物工艺设计的软件包
６.序列格式转换：FASTA与BLAST查询输出文件的处理软件
７.引物分析
８.序列综合分析
９.检索与阅读
１０.基因芯片