实验常用缩写符号中英对照
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AcNPV ,Autographa califorinica nuclear polyhedrosis virus,苜蓿银纹夜蛾核型多角体病毒
att ,attachmet site,接受位点
Apr ,Ampicillin replication sequence,氨卞青霉素抗性基因
ARS ,Autonomors replication sequence,自主复制序列
Adv ,Adenovirus,腺病毒
atRNA ,Antisense RNA,反义RNA
BmNPV ,Bombyx mori nuclear polyhedrosis virus,家蚕核型多角体病毒
Bacmid ,Baculovirus plasmid,杆状病毒质粒
BAS ,Bovine serum albumin,牛血清白蛋白
BAP ,Baceria alkaline phosphase,细菌碱性磷酸酶
bp ,Base pair ,碱基对
BV ,budded virus,出芽病毒
Bt ,Bacillus thuringiensis,苏芸金芽孢杆菌
BsNPV ,Buzura suppressaria NPV,大尺蠖核型多角体病毒
cry ,crystal protein gene,晶体蛋白基因
cDNA ,complementary DNA,互补DNA
cccDNA ,covalent close circle DNA,公价闭合环状DNA
CAP ,calf alkaline phosphase,小牛碱性磷酸酶
CAP ,Catabolic activative protein,分解代谢活化蛋白
Cmr ,Chloramphenicol resistant gene,氯霉素抗性基因
ChDNA ,chromosome DNA,染色体DNA
CEN ,centromer,着丝粒、端粒
CaMN ,Caullinrs mozic virus,花叶菜花叶病毒
CAT ,Chloramphnicol acetyltransferase,氯霉素乙酰转移酶基因
CBP ,Cap binding protein,帽子结合蛋白
DNA ,Deoxy ribonucleic acid,脱氧核糖核酸
ddH2O ,doble distilled water,双蒸水
dsDNA ,Double strand DNA,双链DNA
DTT ,Dithiothreitol,二硫苏糖醇
DNase Ⅰ ,DNA enzyme Ⅰ,DNA酶Ⅰ
DNA pol ,DNA polymerase,DNA聚合酶
ddNTP ,dideoxy NTP ,双脱氧NTP
dsRNA ,double strand RNA,双链RNA
dNTP ,deoxy NTP,脱氧核苷三磷酸
ELISA ,ensyme-linked immunosorbent assay,酶联免疫分析
E.coli ,Esherichia coli,大肠杆菌
EDTA ,ethylenediamine tetraacetic acid,二乙胺四乙酸
EB ,ethedium bromide,溴化乙锭
EBV ,Epstein barr virus,EB病毒
FG ,foregn gene,外源基因
GV ,Granulosis virus,颗粒体病毒
egt ,ecdysteroid UDP-glucosyl transferase gene,蜕皮激素UDP葡萄糖基转移酶基因
eif ,extension initiation factor,延伸起始因子
env ,envelope gene,囊膜糖蛋白基因
exoⅢ ,exonucleaseⅢ,外核酸酶Ⅲ
GFP ,Green fluorescent protein,绿色荧光蛋白(gfp)
gag ,nucleocapsid gene,核衣壳蛋白基因
HBV ,Hepetitis B virus,乙型肝炎病毒
HBeAg ,HBV e antigen,乙型肝炎病毒e抗原
HBcAg ,HBV c antigen,乙型肝炎病毒核心抗原
HBsAg ,HBV s antigen,乙型肝炎病毒表面抗原
HIV ,Human immunodeficiency virus,人免疫缺陷型病毒
HSV ,Human simple virus,单纯疱疹病毒
HCV ,Hepatitis C virus,丙型肝炎病毒
hr ,homologors region,同源重复序列
hnRNA ,Heterologous nuclear RNA,核不均一RNA
int ,Integration enzyme gene,整合酶基因
IS ,initiation site,(转录)起始位点
ITF ,induction transcription factor,诱导转录因子
IE ,immediate early gene,极早期基因
iap ,inbibiting apoptose gene,凋亡抑制基因
Kar ,kanamycin resistant gene,卡那霉素抗性基因
kb ,kilobase pair,千碱基对
IFN ,interferon,干扰素
IPTG ,Isopropyl-β-D-thiogalactopyranoside,异丙醇β-硫代半乳糖苷
LsNPV ,Leucania separata NPV,粘虫核型多角体病毒
LTR ,Long terminal repeat,长末端重复序列
LacZ ,β-galactosidase gene,β-半乳糖苷酶基因Z
Luc ,Luciferase gene,荧光素酶基因
Lef ,late expression factoc gene,晚期表达因子基因
MW ,molecular weight,分子量
mRNA ,messanger RNA,信息RNA
miDNA ,mitochondral DNA,线粒体DNA
msDNA ,multi copy ssDNA,多拷贝单链DNA
MCS multip cloning site,多克隆位点
MOI multipe of infection,感染复数
micRNA ,mRNA enterfering complementaty RNA,干扰mRNA的互补RNA
Ner ,Neomycin resistant gene,新霉素抗性基因
NCS ,non coding sequence,非编码序列
NC nitrocellulose,硝酸纤维素膜
NTP ,Nucleotide triphosphate,核苷三磷酸
ocu ,polyhedrin gene多角体蛋白基因
onc ,cancer gene致癌基因
omp ,outermembrane protein gene,外膜孔蛋白
ORF ,open reading frame,开放阅读框
ori ,origin,复制起始点
PCR ,Polymerase chain reaction,多聚酶链反应
PDH ,pyruvate dehydrogenase,丙酮酸脱氢酶
PEG ,Polyethyleneglycol 聚乙二醇
pfu ,plaque forming units,空斑形成单位
PAGE ,polyacrymide gel eletrophoresis,聚丙烯酰胺凝胶电泳
PE(pe) ,polyhedra envelope,多角体外膜蛋白(基因)
PDV ,polyhedra derived virus,多角体衍生病毒
PRprometer right ,右向启动子
PLprometer left ,左向启动子
RNasin ,RNA enzyme inhibitor,RNA酶抑制剂
RNA ,Ribonucleic acid,核糖核酸
RNase ,RNA enzyme ,RNA酶
rRNA ,Ribosomal RNA,核糖体RNA
RT ,reverse transcriptase,逆转录酶
rNTP ,ribo NTP,核糖核苷三磷酸
RNA pol ,RNA polymerase,RNA聚合酶
SDS ,Sodium dodecyl sulfate,十二烷基硫酸钠
SV40 ,Simian virus 40,猴病毒40
siRNA ,small interfer RNA,短片断干扰RNA
shRNA ,short hairpin RNA,短片断发夹RNA
ssRNA ,single strand RNA,单链RNA
snRNA ,small nuclear RNA,小核RAN
sDNA ,satellite DNA ,卫星DNA
Sf9 ,sodoptera frugiperda 9,昆虫草地夜蛾(细胞)9
Tcr ,Tetracyclin resistant gene,四环素抗性基因
Tn ,Transposon,转座子
TMV ,tobacco mozic virus,烟草花叶病毒
tRNA ,tuansfer RNA,转移RNA
tk ,thymidine kinase gene,胸腺激酶基因
TCID50 ,Tissue culture infective dose,半数组织培养感染剂量
Ti ,tumor inducing plasmid,Ti质粒
UV ,ultraviolet light,紫外光
UTR ,rn-translation region,非翻译区
wt ,wild type,野生型
Xgal ,5-bromo-4-chloro-3-indolyl-β-D-galactoside,5-溴-4-氯,3-吲哚β-D半乳糖苷