【共享】DNA测序常见英文对照(转载)

转载，感谢原作者

DNA测序常见英文单词对照

（1）Quantitative analysis of expression of myocardial CYP11B1 and CYP11B2 genes in rats of two groups Taking 100bp Plus Ladder as Marker,clear amplified strands could be seen at 440bp?461bp and 336bp sites,DNA sequencing proved they were the encoding gene segments of CYP11B1,YP11B2 and GAPDH.

（1）两组大鼠心肌CYP11B1及CYP11B2基因表达的定性分析:以100bpPlusLad-der为Marker,分别在440bp,461bp及336bp处可见清晰的扩增条带,DNA测序证实为CYP11B1,YP11B2及GAPDH的编码基因片段。

（2）Proved by PCR-RFLP and DNA sequencing,the genotype of CYP3A4 *3 was wild type in all subjects.

（2）经PCR-RFLP检测并经DNA测序验证,所有受检者的CYP3Aundefined3基因型均为野生型。

（3）Candidate CHO cell clones were screened by Zeocin, and the results of DNA sequencing showed that pSecTag VEGF165 and pSecTag Ang1 were transformed into CHO cells.

（3）用Zeocin筛选到候选的CHO细胞株,DNA测序结果表明,pSecTag-VEGF165和pSecTag-Ang1已分别成功转化入CHO细胞中。

（4）Rapid and Economic DNA Sequencing Using ABI PRISM~(TM) 310 Sequence

（4）应用ABI PRISM~(TM)310测序仪进行快速且经济的DNA测序

（5）Dna sequence esults(98.11%) detection results of UT-PCR and PCR-RFLP was the same as DNA sequence.

（5）结果UT-PCR和PCR-RFLP的检测结果与DNA测序结果的符合率均为98.11%。

（6）Results of the analysis of DNA sequence approved that the hc γ1 gene fuse with HCV E1 + E2661 or E2661 were cloned into the recombinant plasmids successfully.

（6）DNA测序结果证实插入片段即是HCV E1+E2661及E2661与HC γ1的融合基因;

（7）Methods:The NF2 gene (exon2)mutation in 36 schwannomas (16 acoustic neuromas, 20 other schwannomas) were observed by PCR SSCP and DNA sequence.

（7）方法 :用PCR SSCP、DNA测序检测 36例神经鞘瘤 (听神经瘤 16例 ,其它 2 0例 )中NF2基因外显子 2的突变。

（8）RESULTS:DNA sequence analysis proved correct reconstruction of A53T and A30P.

（8）结果:DNA测序结果证明构建载体插入正确α-突触核蛋白突变体A53T与A30P基因。

（9）METHODS: RKO cells were treated with selective DNA methyltransferase (DNMTs) inhibitor, 5-Aza-2′-deoxycytidine (5-Aza-CdR), for 72 h. Methylation-specific PCR (MSP), T-A clone and DNA sequence analysis were used to detect 5′CpG island methylation status of p16/CDKN2 tumor suppresor gene.

（9）方法:应用特异性DNA甲基转移酶(DNMTs)抑制剂-5-氮-2′-脱氧胞苷(5-Aza-2′-deoxycytidine,5-Aza-CdR)处理肠癌RKO细胞72h,甲基化特异性PCR(methylation-specificPCR,MSP)及DNA测序法分析p16/CDKN2基因CpG岛甲基化状态;

（10）RESULTS:DNA sequence analysis proved correct reconstruction of A53T and A30P.

（10）结果:DNA测序结果证明构建载体插入正确α-突触核蛋白突变体A53T与A30P基因。

（11）METHODS: RKO cells were treated with selective DNA methyltransferase (DNMTs) inhibitor, 5-Aza-2′-deoxycytidine (5-Aza-CdR), for 72 h. Methylation-specific PCR (MSP), T-A clone and DNA sequence analysis were used to detect 5′CpG island methylation status of p16/CDKN2 tumor suppresor gene.

（11）方法:应用特异性DNA甲基转移酶(DNMTs)抑制剂-5-氮-2′-脱氧胞苷(5-Aza-2′-deoxycytidine,5-Aza-CdR)处理肠癌RKO细胞72h,甲基化特异性PCR(methylation-specificPCR,MSP)及DNA测序法分析p16/CDKN2基因CpG岛甲基化状态;

（12）Methods:Two-step PCR-CTPP was utilized for detecting polymorphism of p73 exon 2 G4C14-A4T14 of 180 healthy controls and the same polymorphism was genotyped by PCR-restriction fragment length polymorphism(RFLP) and DNA sequence analysis.

（12）方法:采用两步法PCR-CTPP技术检测180名正常个体p73基因第二外显子G4C14-A4T14多态性,并以PCR-RFLP和DNA测序验证基因分型结果。

（13）DNA sequence analysis showed that sod gene of SeMNPV encoded 151 amino acids. The sequences homology between SeMNPV sod gene and human sodl gene was 50%, and 64% between SeMNPV and LdNPV, 63% between SeMNPV and HaSNPV, HcNPV, BmNPV, 65% between SeMNPV and AcNPV.

（13）DNA测序结果表明SeMNPV-Z的sod基因编码151个氨基酸,与人的sod1基因的核苷酸的同源性为50％,与LdNPV、HaSNPV、HcNPV、AcNPV和BmNPV的sod基因的同源性分别为64％、63％、63％、65％、63％。

（14）Methods: Target gene was got from rat genomic DNA by PCR,then recombined with the expression vector pGEX-4T-1 and its validity was identified using PCR,restricted endonuclease and DNA sequence analysis.

（14）方法:应用聚合酶链技术(PCR),从大鼠基因组中扩增出目的基因,与表达载体pGEX-4T-1重组,采用PCR、限制性内切酶酶切及DNA测序进行鉴定。

（15）The capillary gel electrophoresis technique in DNA sequence determination

（15）DNA测序中的毛细管凝胶电泳技术

（16）The CGE separation technique usually used in DNA Sequence Determination is introduced in detail in this paper.

（16）本文综合介绍DNA测序中常用毛细管凝胶电泳 (CGE)分离技术。

（17）DNA sequence determination didn't find mutation of RG1 genes.

（17）DNA测序未发现RAG1基因有突变发生;