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简介
来源:丁香实验
操作方法
一、材料 1. 缓冲液及溶液 变性溶液(1.5 mol/L NaCl,0.5 mol/L NaOH) HCl ( 0.2 mol/L) 应用于 DNA 脱嘌呤 中和缓冲液(1 mol/L Tris ( pH 7.4),1.5 mol/L NaCl) 中性转移缓冲液(10X SSC) 6X SSC 6X 蔗糖凝胶上样缓冲液 SYBR Gold 或溴化乙锭 2. 酶及缓冲液 适当的
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