提问
提问
我要登录
|免费注册
丁香实验推荐阅读
Genetic Manipulation of Human Embryonic Stem Cells in Serum and Feeder-Free Media

Generic methods for genetic manipulation of human embryonic stem cells (hESCs) are important for both present research and future commercial applications. To date, differences in cell derivation and culture have required independent optimization of transfection and transdu ...

丁香实验推荐阅读
Human-Induced Pluripotent Stem Cells: Derivation, Propagation, and Freezing in Serum- and Feeder Layer-Free Culture Conditions

The recent discovery of genomic reprogramming of human somatic cells to an embryonic stem (ES) cell-like pluripotent state provides a unique opportunity for stem cell research. The reprogrammed cells, named as induced pluripotent stem (iPS) cells, possess many of the properties of ES cells ...

丁香实验推荐阅读
Derivation of Human Embryonic Stem Cell Lines from Vitrified Human Embryos

Human embryonic stem cell lines are usually derived from human embryos that have become excess to clinical needs in assisted reproduction programs, whether because the couple in question has completed their family or because the embryo was found to be clinically unsuitable for transfer d ...

丁香实验推荐阅读
Growth of Human Embryonic Stem Cells Using Derivates of Human Fibroblasts

The majority of human embryonic stem cell (hESC) lines have been derived and grown using mouse or human feeder cells, or using Matrigel�, an animal derivative rich in extracellular matrix (ECM) proteins. However, reliance on feeder layers and animal products limits the manipulation and clin ...

丁香实验推荐阅读
In Vitro Neural Differentiation of Human Embryonic Stem Cells Using a Low-Density Mouse Embryonic Fibroblast Feeder Protocol

Human embryonic stem cells (hESCs) have the capacity to self-renew and to differentiate into all components of the embryonic germ layers (ectoderm, mesoderm, endoderm) and subsequently all cell types that comprise human tissues. HESCs can potentially provide an extraordinary source ...

丁香实验推荐阅读
Optimization of Physiological Xenofree Molecularly Defined Media and Matrices to Maintain Human Embryonic Stem Cell Pluripotency

We describe in this chapter the development of a xenofree molecularly defined medium, SBX, associated with xenofree matrices, to maintain human embryonic stem cell (hESC) pluripotency as determined by phenotypic, functional and TLDA studies. This simple, inexpensive, and more physi ...

丁香实验推荐阅读
Serum-Free and Feeder-Free Culture Expansion of Human Embryonic Stem Cells

Human embryonic stem cells (hESCs) are pluripotent stem cells derived from the inner cell mass of human blastocysts. hESCs have become a great asset to studying human diseases and genetic functions of healthy organisms. The rate at which hESCs are being used in laboratories is exponentially i ...

丁香实验推荐阅读
Monitoring Stemness in Long-Term hESC Cultures by Real-Time PCR

Human embryonic stem cells (hESC) involve long-term cultures that must remain undifferentiated. The real-time PCR (RT-PCR) technique allows the relative quantification of target genes, including undifferentiation and differentiation markers when referred to a housekeep ...

丁香实验推荐阅读
Single Cell Enzymatic Dissociation of Human Embryonic Stem Cells: A Straightforward, Robust, and Standardized Culture Method

The routine culture and expansion of human embryonic stem (hES) cells has been and is still posing a challenge to researchers wishing to take advantage of the cells' unique potential. In contrast to mouse embryonic stem cells, hES cells usually have to be expanded by tedious mechanical microdiss ...

丁香实验推荐阅读
Dissecting Mitosis with Laser Microsurgery and RNAi in Drosophila Cells

Progress from our present understanding of the mechanisms behind mitosis has been compromised by the fact that model systems that were ideal for molecular and genetic studies (such as yeasts, C. elegans, or Drosophila) were not suitable for intracellular micromanipulation. Unfortun ...

丁香实验推荐阅读
Analysing Kinetochore Function in Human Cells: Spindle Checkpoint and Chromosome Congression

During cell division microtubules of the mitotic spindle segregate the duplicated chromosomes into the two daughter cells. Chromosome–microtubule attachment is mediated by kinetochores, multiprotein complexes assembled on specialized regions of the DNA. Kinetochores m ...

丁香实验推荐阅读
Quantitative In Situ Hybridization of Tissue Microarrays

Tissue microarrays enable the rapid histological localization of gene expression in hundreds of archival samples by in situ hybridization. However, the scoring of tissue microarray data may be influenced by intra- and inter-observer variations, and categorizing continuous var ...

丁香实验推荐阅读
Semiquantitative In Situ Hybridization Using Radioactive Probes to Study Gene Expression in Motoneuron Populations

In situ hybridization is a powerful technique for examining changes in mRNA levels. Its primary advantage over the Northern blot and reverse transcription polymerase chain reaction is the ability to localize specific species of mRNA to a particular cell population in a heterocellular s ...

丁香实验推荐阅读
The Use of Combined Immunohistochemical Labeling and In Situ Hybridization to Colocalize mRNA and Protein in Tissue Sections

This chapter explores the combination of a nonradioactive in situ hybridization technique to detect mRNA with an imunnohistochemical labeling technique for use in formalin- fixed, paraffin-embedded, or frozen tissue sections. This technique allows the combination of detecti ...

丁香实验推荐阅读
Histochemical Localization of Apoptosis With In Situ Labeling of Fragmented DNA

Cell death by apoptosis is now recognized widely as an important constituent of cell turnover and disease pathology. Characterized by the cleavage of DNA into oligonucleosome-sized DNA fragments, end-labeling of fragmented DNA often is used as an in situ histological marker of apoptosi ...

丁香实验推荐阅读
Histochemical Localization of Cell Proliferation Using In Situ Hybridization for Histone mRNA

Monoclonal antibodies to proliferation associated antigens have long been used to histologically localize mitogenesis. However, techniques that distinguish cells in the synthetic or S phase have tended to rely on the in vivo incorporation of tritiated thymidine or thymidine anal ...

丁香实验推荐阅读
In Situ Hybridization to Plant Tissues and Chromosomes

In situ hybridization is a basic method in modern plant cell and molecular biology. It is used to locate the chromosomal position of genomic DNA sequences. It is able to determine the patterns of gene transcription in mature tissues and during development. In situ hybridization, in combination w ...

丁香实验推荐阅读
Identification of Transplanted Human Cells in Animal Tissues

The potential of cell and gene therapy has generated extensive interest over the past several years. More recently, identification of stem cells of various types, especially embryonic stem cells, reinforced this interest. Systematic studies are now being launched to define the biology ...

丁香实验推荐阅读
An In Situ Hybridization Technique to Detect Low-Abundance Slug mRNA in Adherent Cultured Cells

In this chapter, we describe a simple and relatively rapid technique for detecting lowabundance slug mRNA in cultured cells. The procedure uses nonradioactive digoxigeninlabeled RNA probes that are more sensitive than deoxyribonucleic probes and simpler to detect than radioact ...

丁香实验推荐阅读
In Situ Hybridization Using Riboprobes on Free-Floating Brain Sections

A method is described for in situ hybridization of riboprobes to free-floating brain sections. Brain sections are hybridized and processed free-floating in buffer, i.e., without attachment to a support such as a slide. To withstand the extra wear compared with sections processed on-slide, t ...

提问
扫一扫
丁香实验小程序二维码
实验小助手
丁香实验公众号二维码
关注公众号
反馈
TOP
打开小程序