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丁香实验推荐阅读
Zn(II)Cyclen Polyacrylamide Gel Electrophoresis for SNP Detection

We introduce a method for the detection of single-nucleotide polymorphisms (SNPs) by polyacrylamide gel electrophoresis (PAGE) with an additive Zn2+–cyclen complex (cyclen is 1,4,7,10-tetraazacyclododecane), called “Zn2+–cyclen–PAGE.” The method is based on the difference in ...

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Temporal Temperature Gradient Electrophoresis for Detection of Single Nucleotide Polymorphisms

The presence of single nucleotide polymorphisms (SNPs) in nuclear DNA and mitochondrial DNA (mtDNA) can be detected using a range of electrophoretic techniques, of which temporal temperature gradient electrophoresis (TTGE) is often the most user-friendly and reproducible. The te ...

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Single Nucleotide Polymorphism Screening with Denaturing Gradient Gel Electrophoresis

Denaturing gradient gel electrophoresis (DGGE) is a powerful technique for identifying DNA sequence-based differences. The method relies on the fact that double-stranded DNA molecules have unique denaturation rates that are based upon the specific nucleotide composition of the ...

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Pyrosequencing for SNP Genotyping

Pyrosequencing is a real-time DNA sequencing method. It is based on the transformation of pyrophosphates, released during DNA elongation by DNA polymerase, into measurable light. During DNA elongation, a single pyrophosphate molecule is released following incorporation of a sing ...

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Scanning Probe and Nanopore DNA Sequencing: Core Techniques and Possibilities

We provide an overview of the current state of research towards DNA sequencing using nanopore and scanning probe techniques. Additionally, we provide methods for the creation of two key experimental platforms for studies relating to nanopore and scanning probe DNA studies: a synthetic n ...

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Next-Generation Sequencing Methods: Impact of Sequencing Accuracy on SNP Discovery

The advent of next-generation sequencing technologies has spurred remarkable progress in the field of genomics. Whereas traditional Sanger sequencing has yielded the first complete human genome sequence, next-generation methods have been able to resequence several human gen ...

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Mining SNPs from DNA Sequence Data; Computational Approaches to SNP Discovery and Analysis

Single nucleotide polymorphisms (SNPs) are the most abundant form of genetic variation and are the basis for most molecular markers. Before these SNPs can be used for direct sequence-based SNP detection or in a derived SNP assay, they need to be identified. For those regions or species where no vali ...

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SNP Databases

Researchers interested in obtaining detailed information on SNPs now work in a golden age of online database availability: never has so much data and such a wealth of information been freely accessible for such a substantial proportion of the 18 million single nucleotide polymorphism (SNP) ...

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Silent (Synonymous) SNPs: Should We Care About Them

One of the surprising findings of the Human Genome Project was that single nucleotide polymorphisms (SNPs), which, by definition, have a minor allele frequency greater than 1%, occur at higher rates than previously suspected. When occurring in the gene coding regions, SNPs can be synonymous (i ...

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SNPs: Impact on Gene Function and Phenotype

Single nucleotide polymorphism (SNP) is the simplest form of DNA variation among individuals. These simple changes can be of transition or transversion type and they occur throughout the genome at a frequency of about one in 1,000bp. They may be responsible for the diversity among individual ...

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High-Throughput SPR Biosensor

Surface plasmon resonance (SPR) imaging technique is label free, real-time, and high-throughput analysis method for interaction studies with array format. The application of SPR imaging for the small molecule arrays, which were fabricated by photoaffinity crosslinking, can be the f ...

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Permeable Cell Assay: A Method for High-Throughput Measurement of Cellular ATP Synthetic Activity

“Permeable Cell Assay” is an efficient method to measure cellular activity of ATP synthesis. Although ATP is a major energy source for biological reactions, it has been difficult to measure cellular ATP synthetic activity quantitatively. In this assay, bioluminescence from the lucife ...

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Drug Discovery Through Functional Screening in the Drosophila Heart

Although advancements in the preventive and therapeutic strategies of cardiac diseases have successfully improved the prognosis of many types of cardiac diseases, they are still challengeable targets because of their high mortality and large medical expenses. Moreover, becau ...

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Novel Methodology for Immobilization of Biomolecules on the Surface of a Photoresponsible Polymer Containing Azobenzene Moiety

We have introduced the principles of a newly created photoimmobilization technology using photoresponsive azopolymers and reviewed its application to the fabrication of immunochip. We can consider two important factors in our approach, the deformation process that is induced by ...

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High-Performance Gene Expression Module Analysis Tool and Its Application to Chemical Toxicity Data

Gene clustering is one of the main themes of data mining approaches in bioinformatics. Although it has the power to analyze gene function, interpretation of the results becomes increasingly difficult when the number of experiments (samples) exceeds hundreds or more. A new type of clustering ...

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Computational Overview of GPCR Gene Universe to Support Reverse Chemical Genomics Study

In order to support high-throughput screening for ligands of G-protein coupled receptors (GPCRs) by using bioinformatics technology, we introduce a database (SEVENS) with genome-scale annotation and software (GRIFFIN) that can simulate GPCR function. SEVENS (http://sevens.c ...

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High-Throughput Construction of ORF Clones for Production of the Recombinant Proteins

Expression-ready cDNA clones, where the open reading frame (ORF) of the gene of interest is placed under the control of an appropriate promoter, are critical for functional characterization of the gene products. To create a resource of human gene products, we attempted to systematically con ...

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Systematic Cloning of an ORFeome Using the Gateway System

With the completion of the genome projects, there are increasing demands on the experimental systems that enable to exploit the entire set of protein-coding open reading frames (ORFs), viz. ORFeome, en masse. Systematic proteomic studies based on cloned ORFeomes are called “reverse prot ...

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ORFeome Cloning

Reverse chemical genomic approach is expected to greatly expedite the discovery of new compounds, which modulate biological phenotypes in various ways. However, toward this end, various contents and platforms must be well prepared in the research community. In this regard, genome-wi ...

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Fully Automated Two-Dimensional Electrophoresis System for High-Throughput Protein Analysis

A fully automated two-dimensional electrophoresis (2DE) system for rapid and reproducible protein analysis is described. 2DE that is a combination of isoelectric focusing (IEF) and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) is widely used for protein ...

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