提问
提问
我要登录
|免费注册
丁香实验推荐阅读
Xenograft Mouse Models for Tumour Targeting

Identification and development of new monoclonal antibodies and other biological molecules such ad Fab fragments, diabodies and peptides is a key focus of many biotechnology and pharmaceutical companies and academic research department in several therapeutic areas, includi ...

丁香实验推荐阅读
Evaluation of Recombinant Antibodies on Protein Microarrays Applying the Multiple Spotting Technique

The generation of recombinant antibodies by phage display in high-throughput demands fast downstream technologies and streamlined processes for the identification and initial characterisation of individual binders. Next to standard immunological methods such as enzym ...

丁香实验推荐阅读
Antibody Microarrays for Expression Analysis

In recent years, antibody microarrays have developed into an important tool for proteomics. As a multiplexing technique, they facilitate the highly parallel detection of hundreds of different analytes from very small sample volumes of only a few microlitres. This is combined with high se ...

丁香实验推荐阅读
Targeted Polymeric Nanoparticles

Hybrid sub-micrometer particles are of great interest and have been extensively applied to tailor the catalytic, electrical, optical, magnetic, mechanical or thermal properties of the material. Especially their application in the field of diagnostics and drug delivery systems is a r ...

丁香实验推荐阅读
Immunoliposomes

In this chapter, we describe the generation of immunoliposomes by means of two different methods applying different scFv formats. The cloning strategies of these scFv variants possessing an additional cysteine residue for site-directted coupling are also presented in this chapter. ...

丁香实验推荐阅读
Transient Production of scFv-Fc Fusion Proteins in Mammalian Cells

Single chain Fv (scFv) antibody fragments fused to the human immunoglobulin G (IgG) Fc moiety obtain IgG-like properties, but nevertheless they are still encoded by a single gene. As transient production of scFv-Fc proteins in mammalians can easily achieve milligram amounts, this antibo ...

丁香实验推荐阅读
Expression of Antibody Fragments in Transgenic Plants

Plants are attractive systems for the expression of full-length antibodies and also for antibody derivatives such as scFvs, diabodies, camelid VHH and minibodies. For ‘Molecular Pharming’ ER retention of antibody fragments has been turned out to be the method of choice for production in le ...

丁香实验推荐阅读
Bioreactor Production of scFv Fragments in Pichia pastoris

The pubmed search term “pastoris AND (express OR produced OR expression OR production)” yielded 877 hits in December 2008, dated from 1987 to 2009. At the same time, the search term “pastoris AND (bioreactor OR fed-batch OR continuous OR fermentations OR large-scale OR fermentation OR pilot)” re ...

丁香实验推荐阅读
Improving Expression of scFv Fragments by Co-expression of Periplasmic Chaperones

Today, recombinant protein expression in E. coli is usually considered a routine technology. Nonetheless, problems with soluble expression, rooted in protein aggregation competing with folding, are still frequently encountered. Many antibody fragments from natural sources ...

丁香实验推荐阅读
Production of Antibodies and Antibody Fragments in Escherichia coli

Antibodies are an important and growing family of approved therapeutic drugs. Antibody fragments of various types as well as more traditional monoclonal antibodies can be produced using E. coli as the production host. This chapter provides protocols for producing antibody fragments ...

丁香实验推荐阅读
Purification and Analysis of Strep-tagged Antibody-Fragments

The Strep-tag is a nine-residue minimal peptide sequence with intrinsic affinity towards streptavidin. This peptide – or its improved version Strep-tag II – can be fused to recombinant antibody fragments and other proteins in various fashions. The fact that the interaction between the St ...

丁香实验推荐阅读
Analysis and Purification of Antibody Fragments Using Protein A, Protein G, and Protein L

Today, monoclonal antibodies (mAbs) form the largest category of biopharmaceuticals in clinical trials and their number is expanding rapidly (DataMonitor 2007). The antibodies or functional antibody fragments are being produced in artificial production systems like mammal ...

丁香实验推荐阅读
Production of Antibody Fragments in the Gram-Positive Bacterium Bacillus megaterium

For many applications antibody fragments like scFv or Fab are an alternative to full size IgGs. These fragments can be produced in bacterial host systems, which are less time and cost intensive than mammalian cell culture, the production host for more complex IgGs. The Gram-negative bacterium ...

丁香实验推荐阅读
Purification and Characterization of His-Tagged Antibody Fragments

Immobilized metal affinity chromatography (IMAC) has become a widely used method for the affinity purification of recombinant proteins and, in particular, of antibody fragments produced in Escherichia coli. IDA-Sepharose and NTA-agarose charged with different divalent cati ...

丁香实验推荐阅读
AntibodyCytokine Fusion Proteins with Members of the TNF-Family

The generation of antibody fusion proteins for target-directed delivery of cytokines constitutes a promising immunotherapeutic approach. The fusion of recombinant antibodies to members of the TNF-family that differ clearly in their soluble and membrane-bound forms for their r ...

丁香实验推荐阅读
Miniantibodies

Recombinant antibodies have become a standard component of research, diagnostics, and therapy. In the development of recombinant antibodies – irrespective of the final format – a monovalent construct is virtually always the first protein to be tested. This is due to the fact that essential ...

丁香实验推荐阅读
Generation of Single-Chain Fv Fragments and Multivalent Derivatives scFv-Fc and scFv-CH3 (Minibodies)

Intermediate-sized antibody fragments, such as scFv-CH3 (minibodies) and scFv-Fc, retain high avidity for their antigen due to their nature of bivalency. In addition, the incorporation of the Fc region enables the generation of antibody fragments with a variety of biological functions ...

丁香实验推荐阅读
Bivalent Diabodies

Single-chain Fv fragments exhibiting monovalent binding can be converted into bivalent molecules by reducing the linker connecting the two variable domains to approximately five residues resulting in homodimerization of two polypeptide chains. This protocol describes the c ...

丁香实验推荐阅读
scFv by Two-Step Cloning

The scFv-chain Fv (scFv) corresponds to the smallest functional binding unit of a classical antibody and constitutes a favourite format for the comparative first evaluation of the recombinant potential of an antibody. In this protocol, a two-step cloning strategy is described followi ...

丁香实验推荐阅读
CDR-FR Peptides

The non-immunogenic antigen binder has emerged as an important component in the research of cell recognition and targeting with the high degree of specificity inherited from prototypical antibody (Hoogenboom 2005; Holliger and Hudson 2005; Hu et al. 1996; Olafsen et al. 2004; Shahied et al. 20 ...

提问
扫一扫
丁香实验小程序二维码
实验小助手
丁香实验公众号二维码
关注公众号
反馈
TOP
打开小程序