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Human Skin Organ Culture

Reepithelialization is the main process during wound healing that specifically defines the reconstruction of the stratified squamous epithelium. In partial-thickness epidermal wounds, reepithelialization arises from viable epidermal cells at the wound edges as well as fr ...

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A Quantifiable In Vitro Model to Assess Effects of PAI-1 Gene Targeting on Epithelial Cell Motility

Gene “knockout” studies and analysis of physiologically relevant in vitro models have clarified basic mechanisms in the tissue response to injury (1-5). Fundamental to this process is the genetic reprogramming required for conversion of normally sedentary cells to an actively motil ...

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The Fibroblast-Populated Collagen Lattice: A Model of Fibroblast Collagen Interactions in Repair

The healing of dermal defects requires the replacement and integration of a new connective-tissue matrix at the repair site. The deposition and organization of that new connective-tissue matrix entails the interaction between fibroblasts and collagen. A better understanding of th ...

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Excisional Wound Healing: An Experimental Approach

Wound healing disorders present a serious clinical problem and are likely to increase since they are associated with diseases such as diabetes, hypertension, and obesity. Additionally, increasing life expectancies will cause more people to face such disorders and further aggravate ...

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Implantable Wound Healing Models and the Determination of Subcutaneous Collagen Deposition in Expanded Polytetrafluoroethylene Implants

Implantable wound-healing models are made of artificial materials and are designed to monitor a systemic or local effect on the wound-healing response induced by a pathological condition or treatment. An ideal implantable model should accurately reproduce a wound, allow multiple as ...

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Particle-Mediated Gene Therapy of Wounds

Advances in molecular biology and the understanding of the molecular basis of many diseases have provided tools necessary for a new approach to the treatment of both inherited and acquired diseases. This approach, called gene therapy, was initially focused on the correction of inherited d ...

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Use of High-Throughput Microarray Membranes for cDNA Analysis of Cutaneous Wound Repair

Hypertrophic scar formation represents an abnormal wound-healing response following thermal injuries or partial-thickness wounds. Specific growth factors, cytokines, extracellular matrix molecules, and proteinases that are known to alter cell proliferation and migr ...

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Isolation, Culture, and Characterization of Human Intestinal Smooth Muscle Cells

In vitro models utilizing human mesenchymal cells isolated from normal and pathological tissue have proven very useful for studies of wound repair and fibrosis. We have been studying the pathogenesis of intestinal fibrosis and, over the course of 20 yr, have refined techniques for the isola ...

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Methods of Measuring Oxygen in Wounds

Accurate measurement of tissue oxygen tension has led to an understanding of the crucial role of oxygen in wound healing and the degree to which oxygen supply in wounded tissue is often the limiting factor in healing. This, in turn, has led to studies demonstrating that activation of the sympathetic ...

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Measurement of Chemokines at the Protein Level in Tissue

The recruitment of specific leukocyte subpopulations in response to injury is a fundamental mechanism of acute and chronic inflammation. The elicitation of leukocytes is dependent on a complex series of events, including reduced leukocyte deformability, endothelial cell acti ...

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Detection of Reactive Oxygen Intermediate Production by Macrophages

The reparative phenomena that follow sterile tissue injury are an ordered sequence of partially overlapping events and include the macrophage as a key cell type in orchestrating the process of repair. Several studies suggest that the wound-derived macrophage acquires a functional ph ...

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Method for Detection and Quantitation of Leukocytes During Wound Healing

The wound repair process is a highly ordered series of events that encompasses hemostasis, inflammatory cell infiltration, tissue regrowth, and remodeling. An important component of normal wound healing is the generation of an inflammatory reaction, which is characterized by the se ...

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Analysis of Collagen Synthesis

The process of wound healing consists of an orderly sequence of events characterized by the specific infiltration of specialized cells into the wound site. The platelets and inflammatory cells are the first cells to arrive, providing key functions and “signals” needed for the influx of conn ...

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Endothelial Cell Migration Assay: A Quantitative Assay for Prediction of In Vivo Biology

Angiogenesis, the growth of new blood vessels from preexisting ones, is one of the essential phenotypes of tumor formation and is also important in a number of normal physiological processes including growth and development (1), wound healing (2), and reproduction (3-5). An inadequate amou ...

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Quantification of Wound Angiogenesis

Angiogenesis occurs during embryonic development and in adult tissues (1). In developmental angiogenesis, the primitive vascular network formed during neovasculogenesis matures to form the circulatory system. In the adult animal, physiological angiogenesis occurs in the o ...

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Wound Healing in Airways In Vivo

The airway epithelium is a target of inflammatory, environmental, and physical stimuli in diseases such as asthma and bronchopulmonary dysplasia. Damage to the epithelium may compromise both the physical barrier and key metabolic functions. Repair involves the migration and sprea ...

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A Porcine Burn Model

Not only are burns a leading cause of accidental death, but they also result in considerable morbidity and disfigurement leading to significant functional and social impairment. Fortunately, most burns are minor and relatively small. As a result, research efforts have largely focused on ...

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A Mouse Model of Burn Wounding and Sepsis

Despite major advances in the treatment of critically injured burn patients, sepsis remains a major obstacle to recovery (1-4). In fact, sepsis is a leading cause of morbidity and mortality in this patient population (5-6). Those who survive the initial injury become increasingly susceptib ...

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A Functional Genomics Approach to Hematopoietic Stem Cell Regulation

Elucidation of the molecular mechanisms that are responsible for regulating the most basic properties of stem cells, self-renewal, and differentiation remains a major challenge in hematopoietic stem cell biology. We have taken a functional genomics approach towards revealing th ...

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Fate Mapping Hematopoietic Lineages in the Xenopus Embryo

Fate maps reveal the normal contribution of various regions of an early embryo to larval and adult structures. This chapter describes the mapping of primitive blood in embryos of the frog Xenopus laevis by the technique of lineage tracing with a fluorescent dextran.

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