Cancer at its root is a genetic disease brought on by genomic alterations that lead to uncontrolled cell division, metastasis, and enhanced cell survival. The study of genomics can address many of the genetic abnormalities associated with cancer and represents a rapidly progressing field. ...

Metabolic pathways and bioenergetics were described in great detail over half a century ago, and during the past decade a resurgence in integrating these cellular processes with other biological properties of the cell, including growth control, protein kinase cascade signaling, cell ...

The azoxymethane (AOM) plus dextran sulfate sodium (DSS)-induced mouse colon carcinogenesis model is a fascinating tool for investigating the pathogenesis and chemoprevention of colitis-associated colorectal cancer. In this two-stage mouse colon cancer model, tumorigen ...

Mouse lung tumor models are widely used in lung cancer chemopreventive studies. Lung cancer is a heterogeneous disease histologically classified as small-cell lung carcinoma (SCLC) and non-small-cell lung carcinoma (NSCLC), which is usually divided into adenocarcinoma, squamo ...

Our laboratory has f cancer prevention using freeze-dried berries, mainly black raspberries, for more than two decades. Berries contain many known agents with chemopreventive potential including certain vitamins, minerals, simple and complex polyphenols, phytosterols, and v ...

Prostate cancer is now diagnosed mostly at an early stage, and therefore, chemopreventive strategies could be useful to prevent further progression of the disease and to reduce the morbidity and mortality due to this malignancy. Here, we have described methods (immunoblotting, immuno ...

Cancer chemoprevention with natural phytochemical compounds is an emerging strategy to prevent, impede, delay, or cure cancer. This chapter reviews the basic methods used to study the cancer chemopreventive potential of dietary phytochemicals acting by activating the transcri ...

Carcinogenesis is a long-term, multifactorial, and multistep process. Dietary phytochemicals can play a significant role in cancer prevention. In this chapter, we describe common protocols to study the role of phytochemicals in chemoprevention and divide the protocols into two typ ...

Targeting specific and multiple cancer genes, signaling proteins, and transcription factors to prevent cancer is now considered to be the most effective means to prevent cancer. Proteins that bind to a specific DNA gene sequence and act to initiate transcription of the distinct protein ge ...

Chemoprevention represents an important part of cancer medicine’s future. The identification of chemopreventive agents holds tremendous promise for reducing the burden of cancer. Currently, 13 agents are FDA approved for the treatment of precancerous lesions or to reduce the risk of ...

This is an exciting era in the development and cross-disciplinary use of new imaging technologies, including single-photon and multiphoton laser scanning microscopy; second- and third-harmonic generation imaging; coherent anti-Raman stokes imaging (CARS); and live-cell, who ...

DNA adduct measurements provide valuable information about DNA damage associated with exposure to specific genotoxicants. N 2-Ethylidene-dGuo, the major DNA adduct formed upon reaction of acetaldehyde with DNA, has been used to investigate mechanisms of alcohol carcinogenes ...

Primary keratinocytes are harvested from the dorsal skin of 7-week-old mice. Euthanized mice are clipped and cleaned with serial washes in povidone iodine and ethanol solutions. The dorsal skin is removed and treated with a mild 32 �C trypsinization to detach the dermis from the epidermis. Ker ...

Metabolomics is a comprehensive profiling tool used to identify qualitatively and quantitatively all the metabolites present in a biological system. As the number of metabolites in a living being is assumed to be around 30,000, it is necessary to use an adequate extraction procedure and a good ...

The structure elucidation of new secondary metabolites derived from marine and terrestrial sources is frequently a challenging task. The hurdles include the ability to isolate stable secondary metabolites of sufficient purity that are often present in

NMR-based biochemical profiling of natural products has become popular due to the development of high-resolution instruments (400 MHz) and cryogenically cooled probes/preamplifiers, by increasing the sensitivity of NMR instruments several fold and reducing instrument no ...

Lipidomics is defined as a comprehensive analysis of all lipids in a biological system (lipidome). Lipid profiling is a fast-growing area of research due to the involvements of lipids incorporated with human diseases such as obesity, Alzheimer’s diseases, and diabetes as well as in drug disco ...

Liquid chromatography-mass spectrometry (LC-MS or HPLC-MS) is an extremely sensitive analytical technique that enables the detection of metabolites with a vast range of chemistries and molecular masses. Extracts from any biological starting material are first fractionated c ...

Fatty acids (FAs) are involved in a wide range of functions in biological systems. It is important to measure the exact amount of fatty acids in biological matrices in order to determine the level of fatty acids and understand the role they play. The ability to quantify fatty acids in various systems, esp ...

Gas chromatography–mass spectrometry (GC–MS) is a highly reproducible and sensitive analytical technique that has had significant use in the area of metabolite profiling. GC–MS is able to detect a wide variety of metabolites, with highly differing chemistries. In general, extracted b ...