提问
提问
我要登录
|免费注册
丁香实验推荐阅读
Assessing Microvessels After Spinal Cord Injury

This chapter focuses on the assessment of spinal cord microvessels which are lost at the injury epicenter within 24 h after spinal cord contusion or compression in adult rats and mice. The penumbral blood vessels undergo angiogenesis during the first and second week possibly contributing to ...

丁香实验推荐阅读
Assessments of Gliogenesis After Spinal Cord Injury

Spinal cord injury (SCI) results in a large proliferative response that serves to restore homeostatis and replenish cellular deficits. Postinjury repair and recovery can be interrogated as a function of the cell fates adopted by progenitors within the lesion. Administration of bromo ...

丁香实验推荐阅读
Anterograde Axonal Tract Tracing

Deficits in sensory and motor function after spinal cord injury (SCI) are attributable primarily to the interruption of long sensory and motor axonal tracts in the spinal cord. How to exquisitely label these tracts within the spinal cord and their connections to the brain or periphery continu ...

丁香实验推荐阅读
High-Speed Video Recording Used for the Analysis of Hand Shaping in Overground Walking, Cylinder Exploration, Skilled Reaching, and Rung Walking in Ra

Hand shaping in the rat (Rattus norvegicus) is adapted to many functions, including walking, climbing, exploration, and skilled manipulation. An understanding of hand movement can provide insights into the neural control of movement and the impairments that result from brain injury. H ...

丁香实验推荐阅读
Automated Gait Analysis Following Spinal Cord Injury

Successful animal models of spinal cord injury (SCI) are dependent on outcome measures sensitive to minute functional differences following injury. Computer-assisted gait analyses provide an objective measurement of numerous gait characteristics, including but not limit ...

丁香实验推荐阅读
Forelimb Functional Assessments of Rats and Mice After Cervical Spinal Cord Injury

Traumatic spinal cord injury (SCI) frequently occurs to the cervical segments. Regaining arm and hand function is the highest priority for persons with tetraplegia after cervical SCI. Given the tremendous impact that therapeutic changes of hand and arm function would have on the quality of ...

丁香实验推荐阅读
General Introduction to Animal Models of Human Conditions

Animal models serve to imitate (patho) physiological states and/or phenotypical characteristics known to occur in target species (usually man but sometimes other species as well). The use of animal models has had and may continue to have a tremendous impact on medical progress. Laboratory ...

丁香实验推荐阅读
Animal Models of Dementia: Ethical Considerations

This chapter aims to encourage scientists and others interested in the use of animal models of disease – specifically, in the study of dementia – to engage in ethical reflection. It opens with a general discussion of the moral acceptability of animal use in research. Three ethical approaches are he ...

丁香实验推荐阅读
The Role of Rodent Models in the Drug Discovery Pipeline for Dementia

Early-onset familial and late-onset dementia of the Alzheimer-type account for the major proportion of cases of dementia and of neurodegenerative diseases in general. The number of affected individuals is likely to grow in the decades to come due to demographic changes and rising life expe ...

丁香实验推荐阅读
Species, Strain, and Gender Issues in the Development and Validation of Animal Models of Dementia

When establishing animal models of Alzheimer’s disease (AD), the aim is to mimic (certain aspects of) the human condition. However, species, strain, and gender specific features interfere with this goal. Only a few species, like primates, dogs, and bears, spontaneously develop histopatho ...

丁香实验推荐阅读
Culture and Characterization of Rat Mesencephalic Dopaminergic Neurons

Dopaminergic neuronal cell degeneration is the principal characteristic feature of the neuropathology of Parkinson’s disease. Cultures of mesencephalic neurons are widely used as a source of dopaminergic neurons for the study of mechanisms implicated in dopaminergic cell dea ...

丁香实验推荐阅读
Central Nervous System Neuron-Glia Co-culture Models

Glial cell activation plays an important role in the pathogenesis of various neurodegenerative disorders. This article presents a protocol for the preparation of cultures consisting of rat embryonic cortical neurons grown in the presence of cortical microglia, in which the glia are pr ...

丁香实验推荐阅读
Culture of Neonatal Rodent Microglia, Astrocytes, and Oligodendrocytes from Cortex and Spinal Cord

The protocol described in this chapter covers the preparation and culture of enriched populations of microglia, astrocytes, and oligodendrocytes from the cortex and spinal cord of neonatal rat and mouse. The procedure is based on the enzymatic digestion of tissue, followed by the culture of a ...

丁香实验推荐阅读
Amyloid -Peptide Neurotoxicity Assay Using Cultured Rat Cortical Neurons

Neurons cultured from rodent central nervous system tissues represent an important tool in the study of neurodegenerative disease mechanisms and neuroregenerative processes, including the survival- and axon growth-promoting properties of neurotrophic factors. This cha ...

丁香实验推荐阅读
Culture of Rodent Cortical and Hippocampal Neurons

Neurons cultured from rodent central nervous system tissues represent an important tool in the study of neurodegenerative disease mechanisms and neuroregenerative processes, including the survival- and axon growth–promoting properties of neurotrophic factors. This cha ...

丁香实验推荐阅读
Isolation and Culture of Neural Progenitor Cells from Rat Postnatal Cerebellum

The mammalian brain contains undifferentiated, mitotically active, and multipotent neural stem/progenitor cells that in vivo contribute new neurons and glia to specific areas of the mature brain. When isolated under the appropriate conditions, these cells maintain in vitro the ab ...

丁香实验推荐阅读
Culture of Rat Cerebellar Granule Neurons and Application to Identify Neuroprotective Agents

In primary culture of the early postnatal cerebellum, glutamatergic granule cells are highly enriched and recapitulate many properties characteristic of developing granule neurons in vivo. For example, withdrawal of K+ from differentiated rat primary cerebellar granule neur ...

丁香实验推荐阅读
Rodent Spinal Cord Injury Model and Application of Neurotrophic Factors for Neuroprotection

Spinal cord injury (SCI) is a serious clinical problem that causes lifetime disabilities to victims and inflicting huge social burden on our society. One of the main lacunae in developing potential therapeutic measures in SCI is a lack of suitable animal models that could be comparable to clini ...

丁香实验推荐阅读
Evaluating Motor Neuron Death in Neonatal Rats Subjected to Sciatic Nerve Lesion

Neonatal sciatic nerve lesion is a useful experimental model for the study of neuronal cell death. Sciatic nerve transection or crush is the most frequently used approach to evaluate motoneuron loss in the lumbar enlargement of the spinal cord. Here we describe and illustrate the surgical pro ...

丁香实验推荐阅读
Brain Microdialysis in Freely Moving Animals

Brain microdialysis is an analytical technique used for the dynamic monitoring of brain neurochemistry in awake, freely moving animals. This technique requires the insertion of a small dialysis catheter, called a microdialysis probe, into a specific brain region, and its perfusion wi ...

提问
扫一扫
丁香实验小程序二维码
实验小助手
丁香实验公众号二维码
关注公众号
反馈
TOP
打开小程序