Morphological Assessments of Global Cerebral Ischemia: Viable Cells
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Global cerebral ischemia (GCI) can cause selective neuronal damage in a variety of brain regions, including striatum, hippocampus, cortex, and so on. Hematoxylin and eosin and Nissl stains are commonly used histological methods for the morphological assessments of the GCI-viable cells. In addition, the immunohistochemical staining of neuronal nuclei or microtubule-associated protein 2 can be applied to identify the survived neurons after the ischemic insult in this disease model. This chapter introduces the sample preparations, staining procedures, and evaluation methods of these stains.
