【分享】nuture pcr protocol 共22篇

Multiplex PCR-based reverse line blot hybridization assay (mPCR/RLB)—a practical epidemiological and diagnostic tool.
Sequence-specific amplification polymorphisms (SSAPs): a multi-locus approach for analyzing transposon insertions.
Single nucleotide polymorphism (SNP) allele frequency estimation in DNA pools using PyrosequencingTM.
Amplification of 5’ end cDNA with ‘new RACE’.
5’ end cDNA amplification using classic RACE.
3’ End cDNA amplification using classic RACE.
Single-strand conformation polymorphism (SSCP) for the analysis of genetic variation.
Gene splicing and mutagenesis by PCR-driven overlap extension.
AFLP-based transcript profiling (cDNA-AFLP) for genome-wide expression analysis.
AFLP technology for DNA fingerprinting
Detecting antigens by quantitative immuno-PCR.
Competitive PCR for precise nucleic acid quantification.
Qualitative PCR–ELISA protocol for the detection and typing of viral genomes
Competitive PCR–ELISA protocols for the quantitative and the standardized detection of viral genomes.
DNA methylation analysis by pyrosequencing
Protocols for the in situ PCR-amplification and detection of mRNA and DNA sequences
MicroRNA detection by northern blotting using locked nucleic acid probes
A rapid micro chromatin immunoprecipitation assay (μChIP)
PCR-amplification of GC-rich regions: ‘slowdown PCR’
Touchdown PCR for increased specificity and sensitivity in PCR amplification
A robust plant RNA isolation method suitable for Affymetrix GeneChip analysis and quantitative real-time RT-PCR
Quantitative RT-PCR gene expression analysis of laser microdissected tissue samples