细菌的核酸抽提

 

DNA Extraction

・         DNA Extraction from Bacteria (Julie B. Wolf,UMBC)
Phenol/chloroform method

・         DNA Extraction From Bacteria (Triton Method) (NWFSC)

• Isolation of Chromosomal DNA from Bacteria (Hancock Lab) 
      

・         Rapid Genomic DNA Isolation from P. Aeruginosa (Goldberg Lab)

・         DNA Extraction from Gram-positive Bacteria (NWFSC)
This procedure was originally developed for Listeria monocytogenes but has worked well with other Gram+ bacteria

・         Genomic DNA isolation from Bacteria (Newman Lab)

・         DNA Extraction from Bacteria (Lazo Lab)
Phenol/chloroform method

・         Genomic DNA Isolation (Jeffrey D. Newman)
Phenol/chloroform method, this procedure does not work well with Gram + cocci

• Isolation of Aspergillus DNA  (Molecular Genetics Network Lab)
   
• Isolation of Aspergillus DNA: QUIAGEN Method (Molecular Genetics Network Lab)
    

・         Isolation and Quantification of Genomic DNA from Mycobacterium tuberculosis (The Northeast Regional RFLP Laboratory for DNA Typing)
This protocol describes a "DNA mini-prep" procedure for the isolation of chromosomal DNA from Mycobacterium tuberculosis and a procedure for quantifying the amount isolated

RNA Extraction

・         Isolation Of RNA From Bacteroids and Bacteria (Lazo Lab)

• Extraction of RNA from Aspergillus: - Guanidin-thiocyanate and CsCl method (Molecular Genetics Network Lab)
    
• Extraction of RNA from Aspergillus: - Guanidin-thiocyanate and LiCl method (Molecular Genetics Network Lab)

Others

・         Pulsed-field Gel Purification of E. coli Restriction Fragments (Stanford DNA Sequence & Technology Center)