求教BLAST结果的问题！SOS！
丁香园论坛
1564
在siRNA序列设计时，将潜在的序列用BLAST进行同源序列搜索时，出来的结果好象总是存在和其它编码序列同源的序列，郁闷！是不是可以只看E值？还是只看score？这个问题好菜！ 我的目的序列是Human P-glycoprotein (MDR1）中的一个片断，请问出现以下的结果如何判断这个目的序列与其它编码序列不是同源的序列？
下面是BLAST的结果： Score E
Sequences producing significant alignments: (bits) Value
gi|187468|gb|M14758.1|HUMMDR1 Homo sapiens P-glycoprotein (... 38 0.028
gi|42741658|ref|NM_000927.3| Homo sapiens ATP-binding casse... 38 0.028
gi|2353263|gb|AF016535.1|AF016535 Homo sapiens P-glycoprote... 38 0.028
gi|10122135|gb|AC005068.2|AC005068 Homo sapiens BAC clone C... 38 0.028
gi|187486|gb|M29437.1|HUMMDR1A16 Human P-glycoprotein (MDR1...38 0.028
gi|4263559|gb|AC004926.2| Homo sapiens PAC clone RP5-910H9 ... 32 1.7
gi|20087135|gb|AC093268.2| Homo sapiens chromosome 5 clone ... 32 1.7
gi|13491261|gb|AC037433.6|AC037433 Homo sapiens chromosome ... 32 1.7
gi|32698793|ref|NG_002806.1| Homo sapiens adlican pseudogen... 30 6.7
gi|31745457|gb|AC138772.4| Homo sapiens BAC clone RP11-1345... 30 6.7
gi|22003982|gb|AC112771.6| Homo sapiens 3 BAC RP11-144C9 (R... 30 6.7
gi|22549584|gb|AC131205.3| Homo sapiens 12 BAC RP11-185H22 ... 30 6.7
gi|11465016|gb|AC078854.16| Homo sapiens 3q BAC RP11-480B17... 30 6.7
gi|29569255|gb|AC138940.3| Homo sapiens chromosome 5 clone ... 30 6.7
gi|29336173|gb|AC140171.3| Homo sapiens chromosome 5 clone ... 30 6.7
gi|3264544|gb|AC004616.1| Homo sapiens X BAC GSHB-536K7 (Ge... 30 6.7
gi|21070670|gb|AC117527.2| Homo sapiens chromosome 5 clone ... 30 6.7
gi|20429550|gb|AC012289.12| Homo sapiens chromosome 18, clo... 30 6.7
gi|21070523|gb|AC092925.8| Homo sapiens 3 BAC RP11-78O22 (R... 30 6.7
gi|27375013|gb|AC137768.2| Homo sapiens 12 BAC RP13-619I2 (... 30 6.7
gi|19807859|gb|AC105753.2| Homo sapiens chromosome 3 clone ... 30 6.7
gi|14042029|dbj|AK027388.1| Homo sapiens cDNA FLJ14482 fis,... 30 6.7
gi|15277300|gb|AC087260.15| Homo sapiens 12p BAC RP11-437F6... 30 6.7
gi|16328312|gb|AC097528.2| Homo sapiens BAC clone RP11-662G... 30 6.7
gi|17998629|gb|AC097374.3| Homo sapiens BAC clone RP11-71B7... 30 6.7
gi|18854958|gb|AC091873.2| Homo sapiens chromosome 5 clone ... 30 6.7
gi|46402325|gb|AF295017.4| Homo sapiens chromosome 8 clone ... 30 6.7
gi|46402322|gb|AC133329.4| Homo sapiens chromosome 8 clone ... 30 6.7
gi|25167192|gb|AC135335.3| Homo sapiens chromosome 18, clon... 30 6.7
gi|18308296|gb|AC099775.2| Homo sapiens chromosome 3 clone ... 30 6.7
gi|13897301|emb|AL390334.4|CNS06C7N Human chromosome 14 DNA... 30 6.7
gi|16973874|emb|AL445203.23| Human DNA sequence from clone ... 30 6.7
gi|17530761|gb|AC090922.4| Homo sapiens chromosome 8, clone... 30 6.7
gi|15668155|gb|AC013409.8| Homo sapiens BAC clone RP11-471D... 30 6.7
gi|15887258|gb|AC008804.7| Homo sapiens chromosome 5 clone ... 30 6.7
gi|6729561|emb|AL049830.3|CNS00009 Human chromosome 14 DNA ... 30 6.7
gi|15022638|gb|AC079308.15| Homo sapiens 3 BAC RP11-57P12 (... 30 6.7
gi|6456827|emb|AL078603.4|HSJ364H10 Human DNA sequence from... 30 6.7
gi|4581358|emb|AL035446.4|HS460G2 Human DNA sequence from c... 30 6.7
gi|8748884|gb|AC011302.3|AC011302 Homo sapiens BAC clone RP... 30 6.7
gi|11611187|emb|AL359402.3|CNS05TEX Human chromosome 14 DNA... 30 6.7
gi|14422271|emb|AL352978.6|CNS05TBX Human chromosome 14 DNA... 30 6.7
gi|14571660|emb|AL160234.3|CNS01RGY Human chromosome 14 DNA... 30 6.7
gi|12582815|emb|AL356015.3|CNS05TD5 Human chromosome 14 DNA... 30 6.7
gi|13160379|emb|AL121808.4|CNS01DSJ Human chromosome 14 DNA... 30 6.7
gi|11691511|emb|AL360085.26| Human DNA sequence from clone ... 30 6.7
gi|11967594|emb|AL450319.4| Human DNA sequence from clone R... 30 6.7
gi|16972861|emb|AL355529.21| Human DNA sequence from clone ... 30 6.7
gi|16973918|emb|AL590652.10| Human DNA sequence from clone ... 30 6.7
gi|12597031|emb|AL136159.11| Human DNA sequence from clone ... 30 6.7
gi|16973850|emb|AL358937.16| Human DNA sequence from clone ... 30 6.7
gi|15384633|emb|AL450464.10| Human DNA sequence from clone ... 30 6.7
gi|34304330|ref|NM_183063.1| Homo sapiens ring finger prote... 30 6.7
gi|34304329|ref|NM_014245.2| Homo sapiens ring finger prote... 30 6.7
gi|34304332|ref|NM_183237.1| Homo sapiens ring finger prote... 30 6.7
gi|47232523|emb|CR381670.5| Human DNA sequence from clone b... 30 6.7
gi|20218583|emb|AL627107.32| Human DNA sequence from clone ... 30 6.7
下面是BLAST的结果： Score E
Sequences producing significant alignments: (bits) Value
gi|187468|gb|M14758.1|HUMMDR1 Homo sapiens P-glycoprotein (... 38 0.028
gi|42741658|ref|NM_000927.3| Homo sapiens ATP-binding casse... 38 0.028
gi|2353263|gb|AF016535.1|AF016535 Homo sapiens P-glycoprote... 38 0.028
gi|10122135|gb|AC005068.2|AC005068 Homo sapiens BAC clone C... 38 0.028
gi|187486|gb|M29437.1|HUMMDR1A16 Human P-glycoprotein (MDR1...38 0.028
gi|4263559|gb|AC004926.2| Homo sapiens PAC clone RP5-910H9 ... 32 1.7
gi|20087135|gb|AC093268.2| Homo sapiens chromosome 5 clone ... 32 1.7
gi|13491261|gb|AC037433.6|AC037433 Homo sapiens chromosome ... 32 1.7
gi|32698793|ref|NG_002806.1| Homo sapiens adlican pseudogen... 30 6.7
gi|31745457|gb|AC138772.4| Homo sapiens BAC clone RP11-1345... 30 6.7
gi|22003982|gb|AC112771.6| Homo sapiens 3 BAC RP11-144C9 (R... 30 6.7
gi|22549584|gb|AC131205.3| Homo sapiens 12 BAC RP11-185H22 ... 30 6.7
gi|11465016|gb|AC078854.16| Homo sapiens 3q BAC RP11-480B17... 30 6.7
gi|29569255|gb|AC138940.3| Homo sapiens chromosome 5 clone ... 30 6.7
gi|29336173|gb|AC140171.3| Homo sapiens chromosome 5 clone ... 30 6.7
gi|3264544|gb|AC004616.1| Homo sapiens X BAC GSHB-536K7 (Ge... 30 6.7
gi|21070670|gb|AC117527.2| Homo sapiens chromosome 5 clone ... 30 6.7
gi|20429550|gb|AC012289.12| Homo sapiens chromosome 18, clo... 30 6.7
gi|21070523|gb|AC092925.8| Homo sapiens 3 BAC RP11-78O22 (R... 30 6.7
gi|27375013|gb|AC137768.2| Homo sapiens 12 BAC RP13-619I2 (... 30 6.7
gi|19807859|gb|AC105753.2| Homo sapiens chromosome 3 clone ... 30 6.7
gi|14042029|dbj|AK027388.1| Homo sapiens cDNA FLJ14482 fis,... 30 6.7
gi|15277300|gb|AC087260.15| Homo sapiens 12p BAC RP11-437F6... 30 6.7
gi|16328312|gb|AC097528.2| Homo sapiens BAC clone RP11-662G... 30 6.7
gi|17998629|gb|AC097374.3| Homo sapiens BAC clone RP11-71B7... 30 6.7
gi|18854958|gb|AC091873.2| Homo sapiens chromosome 5 clone ... 30 6.7
gi|46402325|gb|AF295017.4| Homo sapiens chromosome 8 clone ... 30 6.7
gi|46402322|gb|AC133329.4| Homo sapiens chromosome 8 clone ... 30 6.7
gi|25167192|gb|AC135335.3| Homo sapiens chromosome 18, clon... 30 6.7
gi|18308296|gb|AC099775.2| Homo sapiens chromosome 3 clone ... 30 6.7
gi|13897301|emb|AL390334.4|CNS06C7N Human chromosome 14 DNA... 30 6.7
gi|16973874|emb|AL445203.23| Human DNA sequence from clone ... 30 6.7
gi|17530761|gb|AC090922.4| Homo sapiens chromosome 8, clone... 30 6.7
gi|15668155|gb|AC013409.8| Homo sapiens BAC clone RP11-471D... 30 6.7
gi|15887258|gb|AC008804.7| Homo sapiens chromosome 5 clone ... 30 6.7
gi|6729561|emb|AL049830.3|CNS00009 Human chromosome 14 DNA ... 30 6.7
gi|15022638|gb|AC079308.15| Homo sapiens 3 BAC RP11-57P12 (... 30 6.7
gi|6456827|emb|AL078603.4|HSJ364H10 Human DNA sequence from... 30 6.7
gi|4581358|emb|AL035446.4|HS460G2 Human DNA sequence from c... 30 6.7
gi|8748884|gb|AC011302.3|AC011302 Homo sapiens BAC clone RP... 30 6.7
gi|11611187|emb|AL359402.3|CNS05TEX Human chromosome 14 DNA... 30 6.7
gi|14422271|emb|AL352978.6|CNS05TBX Human chromosome 14 DNA... 30 6.7
gi|14571660|emb|AL160234.3|CNS01RGY Human chromosome 14 DNA... 30 6.7
gi|12582815|emb|AL356015.3|CNS05TD5 Human chromosome 14 DNA... 30 6.7
gi|13160379|emb|AL121808.4|CNS01DSJ Human chromosome 14 DNA... 30 6.7
gi|11691511|emb|AL360085.26| Human DNA sequence from clone ... 30 6.7
gi|11967594|emb|AL450319.4| Human DNA sequence from clone R... 30 6.7
gi|16972861|emb|AL355529.21| Human DNA sequence from clone ... 30 6.7
gi|16973918|emb|AL590652.10| Human DNA sequence from clone ... 30 6.7
gi|12597031|emb|AL136159.11| Human DNA sequence from clone ... 30 6.7
gi|16973850|emb|AL358937.16| Human DNA sequence from clone ... 30 6.7
gi|15384633|emb|AL450464.10| Human DNA sequence from clone ... 30 6.7
gi|34304330|ref|NM_183063.1| Homo sapiens ring finger prote... 30 6.7
gi|34304329|ref|NM_014245.2| Homo sapiens ring finger prote... 30 6.7
gi|34304332|ref|NM_183237.1| Homo sapiens ring finger prote... 30 6.7
gi|47232523|emb|CR381670.5| Human DNA sequence from clone b... 30 6.7
gi|20218583|emb|AL627107.32| Human DNA sequence from clone ... 30 6.7
