丁香实验_LOGO
登录
提问
我要登录
|免费注册
点赞
收藏
wx-share
分享

Purification of DNA Topoisomerase II from Drosophila melanogaster

互联网

455
To characterize properly protein function and enzymatic activity, it is highly desirable to perform experiments with purified protein preparations. This is especially true in the case of topoisomerase II, because many of the enzymatic assays critical to the topoisomerase field (such as DNA cleavage) require enzyme levels in excess of the DNA substrate (1 -5 ). As a result of the high topoisomerase II concentrations routinely used in these assays, even minor contamination by topoisomerase I or other enzymes that affect DNA structure (such as nucleases) may pose significant technical problems or may lead to erroneous conclusions.
提问
扫一扫
丁香实验小程序二维码
实验小助手
丁香实验公众号二维码
扫码领资料
反馈
TOP
打开小程序