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Liquid Chromatography-Mass Spectrometry of Nucleic Acids

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By virtue of its high-resolving capability, short analysis time, and advanced instrumentation high-performance liquid chromatography (HPLC) has become a versatile technique for the separation and characterization of nucleic acids. Among the various chromatographic modes, which have been summarized in several reviews (1 6 ), ion-pair reversed-phase HPLC (IP-RPHPLC) represents the most popular chromatographic technique applicable to the separation of single-and double-stranded DNA molecules.
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