Eukaryotic Cell Labeling and Preparation for 2-D

Two-dimensional polyacrylamide gel electrophoresis (2-DE) provides the ability to resolve and quantify thousands of proteins from an unfractionated eukaryotic cell lysate. The use of 2-DE in studying eukaryotic cells has run the gambit from ozone-stressed Norway spruce needles (1 ), mapping proteins in the differentiation of mouse endoderm, mesoderm, and ectoderm (2 ) to the study of apoptosis (3 ).