Simultaneous Flow Cytometric Detection of DNA and Cellular Protein Molecules

Flow cytometry (FCM) is a sophisticated technique for rapid simultaneous analysis of multiple cellular parameters. The technique is based on the interception of a hydrodynamically focused stream of single cells in suspension by an excitation light beam at high speed. As a result, each cell produces light scattering and fluorescence, proportional to the cell size, shape, structure, and fluorescently labeled cellular components, respectively.