DNA-Binding studies using in vitro synthesized myb proteins
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The synthesis in vitro of DNA-binding proteins, like Myb, offers many advantages over other expression systems, particularly if large amounts of protein are not required and protein modification, including phosphorylation, is preferable. It is a relatively rapid procedure to subclone and express a large number of Myb proteins that have been truncated or mutated at specific amino acid residues.
