【分享】书籍：Real time PCR (2006)
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Publisher: Taylor & Francis
Number Of Pages: 333
Publication Date: 2006-06-13
ISBN-10 / ASIN: 041537734X
ISBN-13 / EAN: 9780415377348
Binding: Paperback
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Book Description:
The BIOS Advanced Methods series is intended for advanced undergraduates, graduate students and established research scientists. Titles in this series are designed to cover current important areas of research in life sciences, and include both theoretical background and detailed protocols. The aim is to give researchers sufficient theory, supported by references, to take the given protocols and adapt them to their particular experimental systems.
Real-time PCR is based on the conventional principles of PCR and, with the simple shift of emphasis from the end-product to the whole course of the PCR process, has established itself as the most sensitive and specific quantitative PCR method. Real-time PCR is, like any other modern method in molecular genetics, expanding, with potential applications even in proteomics.
With a variety of detection chemistries, an increasing number of platforms, multiple choices for analytical methods and the jargon emerging along with these developments, real-time PCR is facing the risk of becoming an intimidating method, especially for beginners. Real-Time PCR provides the basics, explains how they are exploited to run a real-time PCR assay, how the assays are run and where these assays are informative in real life. It doesn't cover every aspect of real-time PCR, but addresses the most practical aspects of the techniques with the emphasis on 'how to do it in the laboratory'. Keeping with the spirit of the Advanced Methods Series, most chapters provide an experimental protocol as an example of a specific assay.
链接更新2008.3.10
http://rapidshare.com/files/87432248/Real-time_PCR.pdf
Table of Contents: 1. An introduction to real-time PCR / Gregory L. Shipley -- 2. Data analysis and reporting / Pamela Scott Adams -- 3. Relative quantification / Michael W. Pfaffl -- 4. Normalization / Jim Huggett, Keertan Dheda and Stephen A. Bustin -- 5. High-throughput primer and probe design / Xiaowei Wang and Brian Seed -- 6. Quantitative analysis of ocular gene expression / Stuart N. Peirson -- 7. Quantitative gene expression by real-time PCR : a complete protocol / Thomas D. Schmittgen -- 8. Real-time PCR using SYBR green / Frederique Ponchel --
9. High-resolution melting analysis for scanning and genotyping / Virginie Dujols, Noriko Kusukawa, Jason T. McKinney, Steven F. Dobrowolsky and Carl T. Wittwer -- 10. Quantitative analyses of DNA methylation / Lin Zhou and James (Jianming) Tang -- 11. Mitochondrial DNA analysis / Steve E. Durham and Patrick F. Chinnery -- 12. Real-time immuno-PCR / Kristina Linda and Mikael Kubista -- 13. Clinical microbiology / Burcu Cakilci and Mehmet Gunduz -- 14. Clinical virology / David M. Whiley and Theo P. Sloots -- 15. Solid organ transplant monitoring / Omaima M. Sabek -- 16. Real-time PCR applications in hematology / Anne M. Sproul --
17. Real-time PCR for prenatal diagnosis of monogenic diseases caused by single nucleotide changes - the example of the hemoglobinopathies / Joanne Traeger-Synodinos, Christina Vrettou and Emmanuel Kanavakis.
Number Of Pages: 333
Publication Date: 2006-06-13
ISBN-10 / ASIN: 041537734X
ISBN-13 / EAN: 9780415377348
Binding: Paperback
--------------------------------------------------------------------------------Book Description:
The BIOS Advanced Methods series is intended for advanced undergraduates, graduate students and established research scientists. Titles in this series are designed to cover current important areas of research in life sciences, and include both theoretical background and detailed protocols. The aim is to give researchers sufficient theory, supported by references, to take the given protocols and adapt them to their particular experimental systems.
Real-time PCR is based on the conventional principles of PCR and, with the simple shift of emphasis from the end-product to the whole course of the PCR process, has established itself as the most sensitive and specific quantitative PCR method. Real-time PCR is, like any other modern method in molecular genetics, expanding, with potential applications even in proteomics.
With a variety of detection chemistries, an increasing number of platforms, multiple choices for analytical methods and the jargon emerging along with these developments, real-time PCR is facing the risk of becoming an intimidating method, especially for beginners. Real-Time PCR provides the basics, explains how they are exploited to run a real-time PCR assay, how the assays are run and where these assays are informative in real life. It doesn't cover every aspect of real-time PCR, but addresses the most practical aspects of the techniques with the emphasis on 'how to do it in the laboratory'. Keeping with the spirit of the Advanced Methods Series, most chapters provide an experimental protocol as an example of a specific assay.
链接更新2008.3.10
http://rapidshare.com/files/87432248/Real-time_PCR.pdf
Table of Contents: 1. An introduction to real-time PCR / Gregory L. Shipley -- 2. Data analysis and reporting / Pamela Scott Adams -- 3. Relative quantification / Michael W. Pfaffl -- 4. Normalization / Jim Huggett, Keertan Dheda and Stephen A. Bustin -- 5. High-throughput primer and probe design / Xiaowei Wang and Brian Seed -- 6. Quantitative analysis of ocular gene expression / Stuart N. Peirson -- 7. Quantitative gene expression by real-time PCR : a complete protocol / Thomas D. Schmittgen -- 8. Real-time PCR using SYBR green / Frederique Ponchel --
9. High-resolution melting analysis for scanning and genotyping / Virginie Dujols, Noriko Kusukawa, Jason T. McKinney, Steven F. Dobrowolsky and Carl T. Wittwer -- 10. Quantitative analyses of DNA methylation / Lin Zhou and James (Jianming) Tang -- 11. Mitochondrial DNA analysis / Steve E. Durham and Patrick F. Chinnery -- 12. Real-time immuno-PCR / Kristina Linda and Mikael Kubista -- 13. Clinical microbiology / Burcu Cakilci and Mehmet Gunduz -- 14. Clinical virology / David M. Whiley and Theo P. Sloots -- 15. Solid organ transplant monitoring / Omaima M. Sabek -- 16. Real-time PCR applications in hematology / Anne M. Sproul --
17. Real-time PCR for prenatal diagnosis of monogenic diseases caused by single nucleotide changes - the example of the hemoglobinopathies / Joanne Traeger-Synodinos, Christina Vrettou and Emmanuel Kanavakis.
