Fluorescent Assessment of Intracellular Calcium in Individual Cells
互联网
274
Calcium is an important intracellular ion involved in numerous cell processes. There are multiple factors that contribute to the release of Ca2+ . Some factors induce release as part of intracellular signaling cascades, while others result in unwanted changes to both basal and inducible Ca2+ levels. The accurate measurement of intracellular Ca2+ is, therefore, an important tool in neurotoxicology for assessing compounds/substances that disrupt Ca2+ homeostasis. Fluorescent, Ca2+ -sensitive probes Indo-1 and Fluo-3 allow for the quantification of intracellular Ca2+ in individual cells to determine what effects neurotoxins have on both basal and stimulus-dependent Ca2+ concentrations.
