Rapid and Efficient One-Tube PCR-Based Mutagenesis Method
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Oligonucleotide-directed mutagenesis techniques are extensively used for studying gene regulation and DNA and protein structure/function relationships. A number of PCR-based mutagenesis methods have been developed recently (1 –8 ). In general, these PCR-based approaches are simpler and faster than classical mutagenesis methods employing in vivo DNA synthesis, and several of them are now widely practiced.
