Degenerate Oligonucleotide-Primed PCR

The amount of genomic DNA available for genetic studies can often be limiting. Degenerated oligonucleotide-primed polymerase chain reaction (DOP-PCR) is an appropriate method for overcoming these limitations by efficiently performing whole genome amplification. The DOP-PCR technique is increasingly being applied for simultaneous amplification of multiple loci in target DNA using oligonucleotide primers of partially degenerate sequences (1 ). Contrary to other PCR-based general amplification methods (Alu-PCR or IRS-PCR), DOP-PCR is a species-independent technique for the amplification of small amounts of DNA.