DH5a和DH10B的区别

DH5α

F- endA1 glnV44 thi-1 recA1 relA1 gyrA96 deoR nupG Φ80dlacZΔM15 Δ(lacZYA-argF)U169, hsdR17(rk- mk), λ–
1. An Hoffman-Berling 1100 strain derivative (Meselson68)
2. nalidixic acid resistant

DH10B
F- endA1 recA1 galU galK deoR nupG rpsL ΔlacX74 Φ80lacZΔM15 araD139 Δ(ara,leu)7697 mcrA Δ(mrr-hsdRMS-mcrBC) λ-
1.suitable for cloning methylated cytosine or adenine containing DNA
2.an MC1061 derivative (Casadaban80). Prepare cells for chemical ansformation with CCMB80 buffer
3.blue/white selection deoR
4.Streptomycin resistant
 

(责任编辑：大汉昆仑王)