Glia-Neuron Sandwich Cocultures: An In Vitro Approach to Evaluate Cell-to-Cell Communication in Neuroinflammation and Neurotoxicity
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Glia-neuron sandwich cocultures are “in vitro” cell systems suitable to evaluate cell-to-cell interactions relaying on the release of soluble factors, such as proinflammatory cytokines. This chapter presents a protocol to obtain a sandwich coculture from primary rat glial cells and hippocampal neurons. Furthermore, it provides details to evaluate the release of tumor necrosis factor-α by a biological assay and neuronal cell survival, as well as examples of practical application in the investigation of neuroinflammation and its impact on neuronal function.
